两种海洋饵料硅藻的超低温保存  

Cryopreservation of Two Marine Diatoms for Mariculture

作　　者：王起华[1] 张恩栋[1] 王冰[1] 程爱华[1] 

机构地区：[1]辽宁师范大学生物系,辽宁大连116029

出　　处：《辽宁师范大学学报（自然科学版）》1999年第4期310-314,共5页Journal of Liaoning Normal University:Natural Science Edition

基　　金：国家自然科学基金!(39470065)

摘　　要：用两步冷冻法对牟氏角剌藻和新月菱形藻进行了超低温保存，研究了影响存活率的内外因素．结果表明，对这两种藻分别选用１０％ 和１５％ ＤＭＳＯ为抗冻保护剂，预冻至－ ４０℃并保持３０ ｍ ｉｎ 后投入液氮，样品化冻后分别在室温及０℃采用较慢的稀释速率去除ＤＭＳＯ时可获得较高的存活率．此外，处于静止期或经过低温驯化的藻细胞也表现出较大的抗冻性．通过优化内外条件，两种藻的存活率分别达到９１．５％ 和６３．１％ ．Chaetoceros muelleri and Nitzschia closterium are preserved in liquid nitrogen by a two step cooling method.The effects of intrinsic and extrinsic factors on the recovery are studied.It is found that the two diatoms could attain high survival rates when 10% and 15% DMSO are used respectively as cryoprotectants,the prefreezing temperature and time are -40℃ and 30 minutes respectively,and the slow dilution method is used to remove DMSO from the thawed samples at room temperature or 0℃ respectively.In addition,the algae in early stationary growth phase or undergoing cold acclimation show high tolerance to freezing.The viability of C. muelleri and N. closterium are 95.5% and 63.1% respectively under the suitable conditions of cryopreservation.

关 键 词：牟氏角刺藻 新月菱形藻 超低温保存 硅藻 饵料 

分 类 号：S963.213[农业科学—水产养殖] Q949.271[农业科学—水产科学]