甲基对硫磷与牛血清白蛋白相互作用的光谱研究  

Study on Spectra of Interaction between Methyl Parathion and Bovine Serum Albumin

作　　者：张艳霞 李芳轶[2] 张洪卫 胥丹平[2] 徐瑶[2] 季晓彤[2] 张朝红[2] 

机构地区：[1]华北石油水电学校,河北任丘062552 [2]辽宁大学环境学院,沈阳110036 [3]铁岭县疾病预防控制中心,辽宁铁岭112000

出　　处：《农药》2011年第7期498-500,503,共4页Agrochemicals

基　　金：辽宁省教育厅自然科学基金(L2010156)

摘　　要：[目的]为了解释有机磷农药—甲基对硫磷对牛血清白蛋白(BSA)的作用与致毒机理。[方法]采用紫外和荧光光谱法研究甲基对硫磷与BSA的相互作用。[结论]甲基对硫磷能与BSA相互作用,其作用程度和方式与浓度、酸度和离子强度等因素有关。甲基对硫磷对BSA的荧光猝灭方式为静态猝灭,通过计算得到结合位点数(n)和结合常数(K),且结合距离(r)小于7 nm。二者的作用力为氢键或范德华力。甲基对硫磷使BSA构象发生变化,且对酪氨酸残基的影响较大。To explain the action and toxicity mechanism of organophosphorus pesticide-methyl parathion on bovine serum albumin（BSA）.[Methods] The interaction between methyl parathion and BSA was investigated by UV-vis and fluorescence spectroscopy.[Conclusions] Methyl parathion could interact with BSA.The degree and mode were related to such factors as concentration,acidity and ionic strength.The quenching mode between methyl parathion and BSA was static quenching.The binding site（n） and constant（K） were both obtained by calculation and the binding distance（r） was less than 7 nm.The binding force was hydrogen bond or van der Waals force.The conformation of BSA was changed by methyl parathion and the influence on the micro-zone of the tyrosine residues was more.

关 键 词：甲基对硫磷 牛血清白蛋白 相互作用 光谱 

分 类 号：R114[医药卫生—卫生毒理学]