瑞香狼毒超临界CO_2萃取物的杀螨毒力及机理初探  

Preliminary Study on Toxicity of Supercritical Extract of Stellera chamaejasme L. to Tetranychus cinnabarinus and its Acaricidal Mechanism

作　　者：梁为[1] 马兰青[1,2] 卜春亚[1,2] 成军[1,2] 靳永胜[1,2] 王有年[1,2] 师光禄[2] 

机构地区：[1]北京林业大学,北京100083 [2]北京农学院农业部都市农业(北方)重点开放实验室,北京102206

出　　处：《中国农学通报》2011年第15期297-300,共4页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金资助项目(30872029);北京市自然科学基金重点项目(6071001);北京市自然科学基金项目(6092007);北京市教委重点项目;北京市教委平台建设项目;北京市属高校人才强教深化计划资助项目(PHR20090516;PHR200906134)

摘　　要：为了测定瑞香狼毒超临界CO2萃取物对朱砂叶螨的触杀毒力,并了解其作用机理,笔者采用玻片浸渍法测定瑞香狼毒超临界CO2萃取物(SCE)对朱砂叶螨的触杀毒力,观察其对朱砂叶螨的致毒症状;结果表明:SCE对朱砂叶螨24h的触杀LC50值为2.411mg/mL;中毒试螨表现出类似神经毒剂的致毒症状,如兴奋、痉挛等。随后测定了SCE对朱砂叶螨神经系统靶标酶(乙酰胆碱酯酶、单胺氧化酶、Na+-K+-ATP酶和Ca2+-Mg2+-ATP酶)活性的影响。结果表明,SCE能够显著抑制试螨乙酰胆碱酯酶、单胺氧化酶、Na+-K+-ATP酶和Ca2+-Mg2+-ATP酶的活性;据此推测,SCE对朱砂叶螨可能具有神经毒性。In order to study the toxicity and possible lethal mechanism of supercritical extract of Stellera chamaejasme L.,the experiment was conducted.In this study,the contact toxicity of supercritical extract of Stellera chamaejasme L.（SCE） to Tetranychus cinnabarinus was tested using slide dip method,and dynamic toxicosis symptoms of SCE-exposed mites were also detailedly observed.The results showed that the contact toxicity LC50 value of SCE to T.cinnabarinus was 2.411 mg/mL at 24th h post-treatment.Some typical neurotoxic symptoms such as excitement and convulsions were observed in SCE-exposed mites.Subsequently,the changes of activities of AChE,MAO,Na＋-K＋-ATPase and Ca2＋-Mg2＋-ATPase after exposed to SCE were assayed.The experiment showed that SCE could significantly inhibit the activities of AChE,MAO,Na＋-K＋-ATPase and Ca2＋-Mg2＋-ATPase.The results led us to believe that SCE may be a neurotoxin.

关 键 词：瑞香狼毒 超临界萃取物 朱砂叶螨 酶活性 神经毒性 

分 类 号：S436.6[农业科学—农业昆虫与害虫防治]