吐烟花的组织培养  

Tissue Culture of Pellionia repons (Lour.) Merr.

作　　者：王雪兵[1] 罗天杰[2] 于旭东[2,3] 罗灿[2] 吴繁花[2] 

机构地区：[1]海南大学园艺园林学院,海南儋州571737 [2]海南大学农学院,海南儋州571737 [3]海南大学热带作物种质资源保护与开发利用教育部重点实验室,海南儋州571737

出　　处：《中国农学通报》2011年第16期99-103,共5页Chinese Agricultural Science Bulletin

基　　金：海南大学教育教学研究课题立项项目(hdjy0920);海南大学2009科研项目(hd09xm55);中国热带农业科学院热带作物品种资源研究所中央级公益性科研院所基本科研业务专项(PZS062-02);海南省热带农业有害生物检测监控重点实验室开放课题基金专项资助项目(PDCTA1001)

摘　　要：以吐烟花茎段为试验材料,研究其表面消毒方法和不同激素配比的培养基对吐烟花愈伤组织诱导、丛生芽扩繁以及幼苗生根的影响。研究结果表明,外植体表面消毒以70%酒精消毒15s,无菌水漂洗1次,0.1%升汞浸泡6min,无菌水漂洗1次后,再用0.1%升汞浸泡6min,无菌水漂洗4次的效果最佳;同时筛选出吐烟花植株再生的较好激素浓度配比的培养基:MS+3.5mg/L6-BA+0.1mg/LNAA适合愈伤组织诱导,MS+2.0mg/L6-BA+0.1mg/LNAA对丛生芽诱导与扩繁效果较好,MS+0.3mg/LNAA有利于生根,移栽成活率达91%,且植株生长良好。The stem segment of Pellionia repons （Lour.） Merr.was used as explants to study the effect of surface sterilization methods and hormone combinations on callus induction,adventitious shoots and plantlet rooting.The best sterilization method was that the explants were surface sterilized with 70% alcohol for 15 s and rinsed one time with sterile water,disinfected with 0.1% mercuric chloride for 6 min and rinsed one time,and then soaked in 0.1% mercuric chloride for 6 min and rinsed four times.Optimal media appeared to be：MS＋3.5 mg/L 6-BA＋0.1 mg/L NAA for callus induction,MS＋2.0 mg/L 6-BA＋0.1 mg/L NAA for shoot induction and multiplication,and MS＋0.3 mg/L NAA for rooting.The survival rate of plants after transplanted to glasshouse was 91% and the plants grew well.

关 键 词：吐烟花 组织培养 诱导培养 扩繁培养 

分 类 号：Q813.12[生物学—生物工程]