抗生素对白菜型油菜愈伤组织诱导的影响  

Effects of Antibiotics on Induction of Brassica rapa Callus

作　　者：裴冬丽[1,2] 耿慧霞[1] 冯翊阳[1] 赵西[1] 李成伟[1,3] 

机构地区：[1]商丘师范学院生命科学系植物与微生物互作重点实验室,河南商丘476000 [2]河南农业大学农学院,郑州450002 [3]周口师范学院生命科学系,河南周口466001

出　　处：《中国农学通报》2011年第16期168-171,共4页Chinese Agricultural Science Bulletin

基　　金：河南省科技发展计划国际合作项目"芸薹类作物次生代谢物合成调控候选基因的鉴定和功能分析"(084300510076);国家自然科学基金"番茄白粉病抗性反应必需基因ShORR-1作用机理分析"(31071807);河南省科技厅基础与前沿技术项目"番茄与白粉病菌互作的蛋白质组学分析"(082300430320)

摘　　要：以白菜型油菜(Brassica rapa)的亚种菜心(Brassica parachinensis)的子叶和下胚轴为外植体,研究了卡那霉素、潮霉素、羧苄青霉素和头孢霉素4种抗生素对菜心子叶和下胚轴愈伤组织诱导和生长的影响,以及羧苄青霉素和头孢霉素对农杆菌LBA4404的抑制效果。结果表明:卡那霉素、潮霉素对菜心子叶和下胚轴愈伤组织的诱导有较强的抑制作用,卡那霉素作为抗性筛选剂,子叶的筛选浓度为10mg/L,下胚轴的筛选浓度为15mg/L,潮霉素作为筛选剂,子叶的筛选浓度为8mg/L,下胚轴的筛选浓度为6mg/L;羧苄青霉素、头孢霉素对菜心子叶和下胚轴的愈伤组织的诱导影响较小,对农杆菌LBA4404有较强的抑菌作用,作为抑菌剂羧苄青霉素、头孢霉素的浓度以200～400mg/L为宜。The effects of four kinds of antibiotics,Kna,Hyg,Carb and Cef on induction of callus were studied using the cotyledon and hypocotyl of Brassica parachinensis of Brassica rapa subspecies as explants,and the effects of Carb and Cef on inhibiting Agrobacterium LBA4404 were also analyzed.The results indicated that the inhibitions of Kna and Hyg on the induction of callus were effective,the ideal selecting pressure of Kna was 10 mg/L in cotyledon and 15 mg/L in hypocotyl while Hyg was 8 mg/L in cotyledon and 6 mg/L in hypocotyl,while the inhibitions of Carb and Cef were slight.Carb and Cef can suppress proliferation of Agrobacterium LBA4404 better,and the concentration of 200-400 mg/L was optional for the remove of bacterial cells.

关 键 词：抗生素 农杆菌 白菜型油菜 愈伤组织 诱导 

分 类 号：S634.3[农业科学—蔬菜学]