骨髓间质干细胞复合脱钙骨基质和骨形态发生蛋白行兔腰椎横突间融合  

Spinal fusion of lumbar intertransverse process in rabbits by using bone marrow stromal stem cells in conjunction with bone morphogenetic protein with freeze-dried demineralized bone matrix as scaffold

作　　者：胡炜[1] 张学利[1] 高欣凤[2] 崔子健[1] 孙天威[1] 

机构地区：[1]南开大学人民医院脊柱外科,天津300121 [2]天津市儿童医院急创外科

出　　处：《中华骨科杂志》2011年第7期800-805,共6页Chinese Journal of Orthopaedics

摘　　要：目的探讨骨髓间质干细胞复合脱钙骨基质和骨形态发生蛋白进行兔腰椎横突问融合的效果。方法体外分离、培养骨髓问质于细胞，复合于脱钙骨基质材料，以骨形态发生蛋白进行成骨化诱导。日本大耳白兔60只，随机分为三组，A组以骨髓间质干细胞＋脱钙骨基质＋骨形态发生蛋白、B组以脱钙骨基质、C组以自体髂骨行L5．e横突间融合。术后8周取腰椎标本，评估植入材料融合情况；行DR片灰度分析，计算成骨密度及成骨面积；行CT扫描三维重建观察横突间融合形态。HE染色分析横突间融合组织结构。结果大体可见A组及C组植人材料与宿主横突融合，融合组织质地硬，形态不规整；B组横突间植入材料大部分被吸收。DR片示A组与C组横突间呈高密度影，成骨密度不均；B组未达到骨性融合。CT示A组与C组融合标本有明显的连续骨痂通过横突问区；B组未见连续骨痂。A组成骨密度和成骨面积均高于B组，与C组无差异。组织学观察示A组与C组大量软骨形成，靠近横突处新生骨小梁形成；B组横突间连接主要为纤维组织。结论骨髓问质干细胞复合脱钙骨基质和骨形态发生蛋白行兔腰椎横突间融合，其成骨能力接近自体髂骨，优于单纯应用脱钙骨基质。Objective To discuss spinal fusion effect of lumbar intertransverse process in rabbits by using bone marrow stromal stem cells （MSCs） in conjunction with bone morphogenetic protein （BMP） and freeze-dried demineralized bone matrix （FDBM） as scaffold. Methods To separate and cultivate MSCs in vitro, with FDBM as scaffold, osteogenesis was induced by BMP. Sixty Japan white rabbits were randomly divided into three groups. Group A was MSCs＋FDBM＋BMP, group B was FDBM, group C was autogenous ilium cancellous bone （AIB）. Lumbar intertransverse process was fused in lumbar five to six. Rabbits were killed at 8 weeks after operation. The general observation and imageology were used to assess the fusion condition. To estimate the implantation using DR image. Gray scale analysis of DR image caculated the osteogenic density and acreage. The CT scan and three-dimensional reconstruction was used to observe the fusion configuration of lumbar intertransverse process. Results The fusion mass character in group A and C was hard, the morphology was not regulation. The fusion mass was almost absorbed in group B beside little tissue approach transverse process. There were high density image between intertransverse processes, osteogenic density were nonuniform in group A and C. There were nonunion in group B. Consistent callus were existed between intertransverse process in group A and C. There were not consistent callus in group B. Group A was similar to group C in osteogenesis density and acreage. Group B was the worst. There were cartilage and newly born bone trabecular formation in group A and group C. Between transverse process were mainly fiber tissue in group B. Conclusion MSCs in conjunction with BMP and FDBM has the similar osteogenic capability to the AIB and better osteogenic capability than that of FDBM alone when spinal fusion of lumbar intertransverse process is performed in rabbits.

关 键 词：间质干细胞 骨基质 骨形态发生蛋白类 组织工程 

分 类 号：R318.08[医药卫生—生物医学工程]