中草药复方制剂对猪口蹄疫免疫过程中NK细胞的影响  

Effect of Chinese Herb Complex on NK Cells in the Foot and Mouth Disease Immune Process of Pig

作　　者：李冰[1] 孙永祥[2] 李惠兰[2] 李凤元[2] 李永洲[2] 于金玲[1] 徐鹏[1] 刘刚[1] 刘孝刚[1] 

机构地区：[1]辽宁医学院畜牧兽医学院,辽宁锦州121001 [2]辽宁省重大动物疫病应急中心,沈阳110161

出　　处：《中国农学通报》2011年第17期26-30,共5页Chinese Agricultural Science Bulletin

基　　金：辽宁省重大动物疫病应急中心资助项目"重大动物疫病免疫增强技术研究与应用"

摘　　要：将70头30日龄仔猪随机分成7组,每组10头,在口蹄疫免疫过程中按1%添加剂量使用中草药复方制剂,Ⅰ、Ⅱ、Ⅲ组分别在免疫前5天、3天、当天开始连续饲喂3天,Ⅳ、Ⅴ、Ⅵ组分别在免疫前5天、3天、当天饲喂1天。对照组(Ⅶ组)不喂药。分别于免疫前1天和免疫后14天、21天、28天和二免后28天,采血检测NK细胞数量。结果表明:投药组NK细胞数量均高于对照组,Ⅰ、Ⅱ、Ⅲ组NK细胞数量高于Ⅳ、Ⅴ、Ⅵ组,Ⅱ组NK细胞数量最高,其中Ⅰ、Ⅱ、Ⅲ组与对照组差异显著(P<0.05),Ⅳ、Ⅴ、Ⅵ组与对照组差异不显著(P>0.05)。70 piglets which were in the same age were randomly allotted into 7 groups. Pigs in group Ⅰ, Ⅱ, Ⅲ, Ⅳ, Ⅴ, Ⅵ were fed a basal diet supplemented with 1% Chinese herb complex respectively; while animals in group Ⅶ were fed the basal diet only as a control in the foot and mouth disease immune process. Pigs in group Ⅰ, Ⅱ, Ⅲ were immune before 5 days, 3 days, the same day continuous feeding 3 days; group Ⅳ, Ⅴ, Ⅵ were immune before 5 days, 3 days, the same day of feeding one day. These pigs were collected Blood samples and detected NK cells on the day before immunization, 14 days, 21 days, 28 days after immunization and the 28 days after second immunization. The results showed that NK cells in dosage group were higher than control group. NK cells of group Ⅰ, Ⅱ, Ⅲ were higher than group Ⅳ, Ⅴ, Ⅵ NK cells in group Ⅱ were the highest. There was a statistical difference of pig’s NK cells in group Ⅰ, Ⅱ, Ⅲ and control group （P0.05）.

关 键 词：中草药 NK细胞 口蹄疫 免疫 猪 

分 类 号：S852.4[农业科学—基础兽医学]