长春地区医院就诊患者隐孢子虫感染的血清学检测  

Serological Detection of Cryptosporidium spp.Infection in Outpatients in Changchun

作　　者：宋军澎[1] 赵继学[2] 高虹[3] 刘妍[4] 岳红霞[5] 张静[1] 苏艳[1] 尹继刚[1] 

机构地区：[1]人兽共患病研究教育部重点实验室,吉林大学人兽共患病研究所,长春130062 [2]吉林大学第一医院,长春130021 [3]吉林大学第三医院,长春130031 [4]天津工业大学外国语学院,天津300387 [5]吉林大学第二医院,长春130041

出　　处：《中国寄生虫学与寄生虫病杂志》2011年第3期239-240,F0003,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：国家“十一五”传染病重大专项(No.2008ZX10004-011)~~

摘　　要：表达并纯化微小隐孢子虫表面抗原CP23重组蛋白,蛋白质印迹(Western blotting)分析其免疫反应性。采集2010年8～11月份长春市部分医院的各年龄段患者血清,用间接ELISA法检测血清中抗CP23的IgG抗体。Westernblotting分析结果显示,重组蛋白CP23能被感染微小隐孢子虫的牛血清和人阳性血清识别,不能被感染日本血吸虫的小鼠血清、恶性疟患者血清和人阴性血清识别。共采集血清2 046份,总阳性率为3.2%(65/2 046),男性和女性阳性率分别为2.7%(28/1 036)和3.7%(37/1 010),性别间差异无统计学意义(P>0.05)。不同年龄段人群的阳性率差异有统计学意义(P<0.05),71～80岁年龄段的阳性率最高(8.6%,13/152)。CP23 gene of Cryptosporidium parvum was expressed in Escherichia coli,and the recombinant protein was purified.Its immunoreactivity was analyzed by Western blotting.Serum samples were collected from outpatients of different ages from August to November,2010 in Changchun.Indirect ELISA was established to detect the anti-CP23 IgG in sera.Western blotting analysis indicated that the recombinant CP23 protein was recognized by sera from Cryptosporidium parvum-infected calves and positive human sera,but not recognized by sera of mice infected with Schistosoma japanicum,sera from falciparum malaria patients and negetive human sera.The overall anti-CP23 IgG positive rate was 3.2%（65/2 046）.The seropositive rate was 2.7%（28/1 036） in men and 3.7%（37/1 010） in women（P0.05）.The seropositive rates were significantly different among age groups（P0.05）,and the age group of 71-80 had the highest positive rate（8.6%,13/152）.

关 键 词：隐孢子虫 CP23 ELISA 

分 类 号：R382.3[医药卫生—医学寄生虫学]