骨髓间充质干细胞移植促进移植气管上皮再生  

The effect of BMSCs transplantation combined with cryopreservation on trachea allograft epithelium regeneration

作　　者：韩云[1] 纪子钊[1] 蓝妮[2] 庞超见[2] 佟晓杰[2] 石文君[1] 

机构地区：[1]中国医科大学附属盛京医院胸外科,沈阳110004 [2]中国医科大学基础医学院解剖教研室

出　　处：《中华胸心血管外科杂志》2011年第6期365-367,364,共4页Chinese Journal of Thoracic and Cardiovascular Surgery

基　　金：本课题受辽宁省教育厅科学技术研究项目（12010606）资助

摘　　要：目的探讨经静脉移植的骨髓间充质干细胞（BMSCs）对深低温处理的同种异体气管移植后的存活和上皮再生作用。方法用深低温冻储2周和6周的大鼠气管进行同种异体气管移植后，将PKH-26标记后的3～5代BMSCs经鼠尾静脉移植人受体内，通过观察局部PKH-26荧光检测和供体气管的组织学、上皮爬行和再生情况，评价BMSC对移植气管的新生上皮再生作用。结果骨髓间充质干细胞移植并深低温冻储后的移植气管结构完整，组织学检查管腔内为假复层纤毛柱状上皮所覆盖，软骨无变性坏死；术后均能长期存活。PKH-26标记的BMSCs在受损气管组织定植后呈现红色荧光。结论BMSCs可迁移并修复受损组织，并通过促进移植气管上皮再生，从而促进气管的损伤恢复。Objective To investigate the role of BMSCs on enhancing the implant survival and tracheal epithelium regeneration. Methods After transplanted with cryopreserved 2 weeks and 6 weeks allocraft, PHK-26 labeled 3-5 passage BMSCs were injected into the recipient rats via tail vein. Rats in the control groups were injected with the same amount of PBS. We observed the histology of the transplanted trachea including epithelium growth and regeneration, and the PKH-26 fluorescence levels at the para-anastomotic trachea to evaluate the role of BMSC transplantation on the epithelium regeneration. Resuits Rats from BMSCs injection group survived a long period. Histological observation shewed that the tracheal lumen was covered by psudo-striated ciliated columnar epithelium. The cartilage structure was intact. There are no signs of denaturation and necrosis. In the PBS injection group, epithelium regeneration is better in PBS-6-week group than PBS-2-week group. The longest survival time in PBS-6-week group was 32 days, whereas it was 10 days in PBS-2-week group. In BMSCs injection group, rats in BMSC-6-week groups survived longer than 8-week group（ 12 rats were terminated at 1 week, 4 weeks and 8 weeks as planned）. There was one rat who survived and were terminated at the designated 8 weeks time point （ there were 8 rats terminated at 1 week 他 4 weeks as planned）. Other 3 rats died at 30, 32 and 42 days, respectively. The morphology of regenerated epithelium was similar in the two BMSC transplanted groups. PKH-26 labeled BMSCs migrated to the implant site and showed red fluorescence, with most red fluorescence shown at the anastomotic part. Conclusion BMSCs can migrate to the impaired tissue to repair it. BMSCs may exert their reparation function via enhancing epithelium regeneration.

关 键 词：干细胞 气管 移植 同种 低温保存 

分 类 号：R653[医药卫生—外科学]