降糖补肾方对糖尿病大鼠IKKβ mRNA表达的影响  

Influence of Jiang Tang Bu Shen Fang on expression of IKKβ mRNA in diabetic rats

作　　者：范冠杰[1] 孙晓泽[2] 唐咸玉[1] 孙璐[2] 曾星[3] 罗广波[2] 卢绮韵[1] 温建炫[2] 

机构地区：[1]广东省中医院内分泌科,广东510120 [2]广州中医药大学 [3]广东省中医院中心实验室

出　　处：《北京中医药大学学报》2011年第6期402-404,共3页Journal of Beijing University of Traditional Chinese Medicine

基　　金：广东省科技厅资助项目(No.2009B060700122)

摘　　要：目的观察降糖补肾方对小剂量链脲佐菌素(STZ)加高脂饲料诱导的糖尿病模型大鼠肌肉组织IKK激酶β(IKKβ)信使核糖核酸(mRNA)表达的影响。方法雄性SD大鼠高脂饲料喂养4周后注射小剂量STZ,1周后测大鼠空腹血糖,血糖值>11.1 mmol/L者为造模成功的大鼠,将造模成功的大鼠随机分为模型组和降糖补肾方干预组,另设阿司匹林对照组和空白对照组,药物干预4周,取大腿肌肉组织,采用聚合酶链反应逆转录RT-PCR技术检测IKKβmRNA表达水平。结果模型组IKKβmRNA表达明显高于空白对照组,是空白对照组的5.098倍(P<0.01);阿司匹林组、降糖补肾高、中剂量组能明显降低IKKβmRNA表达,分别是空白对照组的2.814、3.246、3.770倍,与模型组比P<0.01;降糖补肾低剂量组能降低IKKβmRNA表达,是空白对照组的4.974倍,与模型组比P>0.05。结论降糖补肾方在一定程度上能抑制糖尿病大鼠IKKβmRNA基因,这可能是降糖补肾方抑制炎症信号通路的传导而发挥其改善胰岛素抵抗治疗糖尿病的机制之一。Objective To observe the influence of Jiang Tang Bu Shen Fang on the expression of I kappa β kinase （IKKβ） mRNA in muscular tissue in diabetic rats induced by low-dose streptozotocin （STZ） and high-fat diet （ HFD ）. Methods Male SD rats were fed HFD for 4 weeks, and then injected low-dose STZ. After one week the level of fasting plasma glucose was detected in rats and those with higher than 11.1 mmol/L of level of fasting plasma glucose were successfully modeled. The rats were randomly divided into the model group, low-dose, mid-dose and high-dose Jiang Tang Bu Shen Fang groups （low-dose, mid-dose and high-dose groups）, aspirin group and blank group. All groups were intervened by relevant medicinal for 4 weeks. The expression of IKKβ mRNA in muscular tissue was detected by using reverse transcription polymerase chain reaction （RT-PCR）. Results The expression of IKKβ mRNA in the model group was significantly higher than that in the blank group （5. 098 times, P 〈0.01 ）. The expression of IKKβ mRNA in the aspirin group, high-dose and mid-dose groups was 2. 814 times, 3. 246 times or 3. 770 times, respectively lower than that in the blank group and showed P 〈 0. 01 compared with that in the model group. In the low-dose group, the expression of IKKβ mRNA was 4. 974 times lower than that in the blank group and showed P 〉 0. 05 compared with that in the model group. Conclusion Jiang Tang Bu Shen Fang can inhibit IKKβ mRNA to some extent in diabetic rats, which may be one of mechanisms of the formula in the treatment of diabetes by inhibiting the conduction of inflammation signal pathway and improving insulin resistance.

关 键 词：降糖补肾方 糖尿病大鼠 IKK激酶β 

分 类 号：R285.5[医药卫生—中药学]