基于炒制原理的决明子饮片质量评价研究  

Quality evaluation of decocting pieces of Juemingzi(Semen Casiae) based on processing principle

作　　者：李丽[1] 张村[1] 肖永庆[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700

出　　处：《北京中医药大学学报》2011年第6期413-416,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家"十一五"科技支撑计划资助项目(No.2006BAI09B06-01-1);综合性中药新药研究开发技术大平台资助项目(No.2009ZX09301-005-03-2)

摘　　要：目的基于决明子炒制原理,研究决明子饮片的质量评价方法。方法以薄层层析法(TLC)及高效液相色谱法(HPLC)指纹图谱定性鉴别5个炮制前后变化显著的成分定量,进行决明子生、炒饮片的质量评价标准研究。结果以正丁醇-冰醋酸-水为展开系统的TLC鉴别方法,可以简便、直观地用于生、炒决明子饮片的鉴别。决明子饮片2个检测波长的指纹图谱,2个萘并吡喃酮苷及3个蒽醌苷元的含量变化均可反映出饮片炮制前后主要化学成分的变化情况。结论中药饮片炮制后,其内在物质基础发生了本质的改变,应逐步建立完善以传统经验鉴别与指纹图谱定性多指标成分定量的具有饮片个性特色的质量评价标准,以实现同一中药不同炮制品的科学评价。Objective To study the quality evaluation method of decocting pieces of Juemingzi （Semen Casiae） based on the processing principle of the decocting pieces. Methods The study on the quality evaluation standard for the crude pieces and stir-fried pieces of Juemingzi was conducted based on the quality identification by TCL and HPLC fingerprints and quantitative determination according to the significant changes of 5 constituents before and after processing. Results The method of TLC taken buranol-acetic acid-water as opening system was used simply and intuitively in the identification of the crude pieces and stir-fried pieces of Juemingzi. The changes of main chemical compositions of decocting pieces of Juemingzi before and after processing were reflected by 2 fingerprints of detecting wavelength and content changes of 2 naphtha-pyrone glycosides and 3 anthraquinone aglycons. Conclusion After processing, the internal material basis will change essentially in decocting pieces. The quality evaluation standard should be established and perfected gradually, which is identified by traditional experience combining with quality determination of fingerprints, quantitative determination of multi-index constituents and characters of decocting pieces aiming at the scientific evaluation of different processing products of the same Chinese medicinal.

关 键 词：炮制原理 决明子 质量评价 

分 类 号：R283.3[医药卫生—中药学]