2株真菌修复石油污染土壤及对植物幼苗生长的影响  

Bioremediation of petroleum-contaminated soil by two strains of fungi and its effect on physiological parameters of plant seedling

作　　者：李德生[1] 高晶[1] 秦晓[1] 李勇正 唐景春[3] 张清敏[3] 

机构地区：[1]天津理工大学环境科学与安全工程学院,天津300384 [2]天津市林果服务站,天津300100 [3]南开大学环境科学与工程学院,天津300071

出　　处：《天津理工大学学报》2011年第3期84-88,共5页Journal of Tianjin University of Technology

基　　金：天津市农业科技成果转化与推广项目(0801160);环境污染过程与基准教育部重点实验室开放基金;国家高技术研究发展计划项目(2007AA061201);中国科学院知识创新工程项目(kzcx1-yw-06-03)

摘　　要：从石油污染的盐碱土壤中分离出具有降解石油性能的两株真菌,将其制成混合菌剂.通过掺混一定比例的石油污染原状土盆栽实验,以石油烃降解率、土壤脱氢酶活性和土壤的微生物多样性等为指标,测定了不同培养时间内各指标的变化.实验结果表明:受试土壤的总石油烃含量为21 000 mg/kg的情况下,加入5%菌剂后第56 d,真菌降解石油的效率达33.13%.在水培条件下,设置两类处理,即单独种植植物(分别为紫花苜蓿、燕麦)、微生物分别与两种植物组合,以表征植物根系活力的根脱氢酶活性和表征叶片脂质过氧化的丙二醛(MDA)含量为指标,研究了真菌促进植物的生长的作用.结果表明:添加5%菌剂在一定程度上提高了植物的根系活力,明显促进了植物生长.Two strains of fungi which were isolated from the petroleum polluted salt alkaloid soil with the petroleum degeneration ability were mixed with humic acid to form the microbial inoculum.Through the pot experiment,the microbial inoculum degeneration capacity was tested by monitoring the petroleum hydrocarbon degeneration rate,the soil dehydrogenation enzyme activity and soil microbial diversity periodly.The results showed that in the case of tested the soil total petroleum hydrocarbon content of 21 000 mg/kg,adding 5% microbial inoculum in the experimented soil pot,the total concentration of petroleum hydrocarbon decreased by 33.13% after 56 days of incubation.In the water cultivating experiment,two types of treatment was designed,that is,plants grown alone（Alfalfa,Canada oats） and two kinds of microorganisms combined with plants respectively.The root dehydrogenation enzyme activity which representing the plant root system vigor and malonydialdehyde（MDA） content in leaves were tested to study promoting effect on plant grow by fungus.The result indicated that with adding 5% microbial inoculum,plant growth were significantly promoted and plant root activity was increased.

关 键 词：石油降解真菌 植物根系活力 丙二醛 紫花苜蓿 燕麦 

分 类 号：X53[环境科学与工程—环境工程]