TNF-α在脊髓背角参与慢性疼痛机制的研究  

作　　者：高峰[1] 赵欣[1] 史瑞红[1] 杨小荣[1] 

机构地区：[1]山西医科大学生理学系,太原030001

出　　处：《中国医疗前沿》2011年第10期15-16,6,共3页China Healthcare Innovation

摘　　要：目的外周伤害性信息传入时,神经胶质细胞会释放以TNF-α为代表的一些致炎因子,并在慢性疼痛的发生、发展过程中起到重要的作用。NR1是构成NMDA受体的一个重要亚基,一般认为NR1磷酸化(p-NR1)表达上调是突触传递发生可塑性变化的基础。在脊髓TNF-α是否通过上调p-NR1的表达导致慢性疼痛的发生和维持目前还不十分清楚。本研究的目的是观察TNF-α释放与大鼠热痛反应以及脊髓背角p-NR1表达间的关系。方法应用PWL检测对大鼠热痛反应变化进行检测,应用Western Blot方法对大鼠腰膨大处脊髓背角的p-NR1表达情况进行检测。结果脚掌注射CFA诱导的炎性痛可以上调脊髓背角p-NR1的表达,缩短热痛反应潜伏期,而鞘内给予TNF-α抗体anti-rTNF-α可以缓解痛觉过敏并下调脊髓背角p-NR1的表达。结论炎性痛时,脊髓中的TNF-α通过上调背角p-NR1的表达,介导了慢性疼痛的发生和发展。Objective Following the input of peripheral nociceptive information,some proinflammatory cytokines from microglia,such as TNF-α,are released,and further produce and maintain the chronic pain.Generally,NR1 is a necessary subunit of NMDA receptor,and the increasing p-NR1（phosphorylation of NR1） can upregulate the plasticity of synaptic transmission.However,it is not yet clear if the increasing TNF-α mediates the chronic pain by upregulating p-NR1 expression in spinal dorsal horn.Therefore,the present study was undertaken to determine whether TNF-α could increase the phosphoralation of NR-1 in spinal cord,thereby chronic pain developed in rats.Methods PWL testing was implemented to observe the thermal response of rats,meanwhile the p-NR1 expression in spinal dorsal horn was measured by Western Blot testing.Results Tthe p-NR1 expression in spinal dorsal horn was upregulated and PWL（paw withdrawal laterncy） decreased in the CFA-injected rats,which both were reversed by TNF-α antagonist anti-rTNF-α.Conclusion All these results revealed that TNF-α produced and maintained the chronic pain by upregulating spinal p-NR1 expression in rats.

关 键 词：TNF-Α p-NR1 慢性疼痛 脊髓背角 

分 类 号：R338[医药卫生—人体生理学]