通过农杆菌介导法将兔防御素NP-1基因导入毛白杨(P.tomentosa)  

Introduction of Rabbit Defensin NP-1 Gene into Poplar (P. tomentosa) by Agrobacterium-mediated Transformation

作　　者：赵世民[1] 祖国诚[2] 刘根齐[1] 黄敏仁[2] 徐金相 孙勇如[1] 

机构地区：[1]中国科学院遗传研究所,本100101 [2]南京林业大学,南京210037

出　　处：《Acta Genetica Sinica》1999年第6期711-714,共4页

基　　金：国家自然科学基金!39770627

摘　　要：采用叶盘法将兔防御素NP－1基因导入毛白杨后，经PCR分析、Southern杂交检测与筛选获得了转基因植株。并经体外抑菌实验表明，转NP－1基因毛白杨植株组织提取物对某些微生物的生长具有明显的抑制作用。Rabbit defensin NP-1 possesses a broad resistant spectrum to pathogens. In this work, we have transferred the NP-1 gene into poplar plants by Agrobacterium-mediated transformation. PCR amplification and Southern analysis showed that rabbit defensin NP-1 gene was integrated into the poplar genome. The transformahon efficiency is about 15.6%. Antimicrobial achvity test showed that the extract of transgenic plants inhibited the growth of the tested microbes.

关 键 词：防御素基因 叶盘法 毛白杨 转基因植株 兔 

分 类 号：S792.117[农业科学—林木遗传育种] Q785[农业科学—林学]