大鼠动脉钙超负荷模型的建立及确证  

Induction and validation of arterial calcium overload in rats

作　　者：孙安阳[1] 俞彰[2] 钟慈声[2] 王永铭[3] 杨藻宸[3] 

机构地区：[1]上海医科大学医学神经生物学国家重点实验室,200032 [2]上海医科大学医学神经生物学国家生物物理学教研室,200032 [3]上海医科大学医学神经生物学国家药理学教研室,200032

出　　处：《中华医学杂志》1999年第10期769-772,共4页National Medical Journal of China

基　　金：国家自然科学基金!39400164

摘　　要：目的建立大鼠动脉钙超负荷（ACO）模型，研究其病理特点。方法分别用原子吸收分光光度法和电子探针微分析法测定模型大鼠血管总钙和平滑肌细胞内钙含量;用扫描电镜和透射电镜观察动脉壁内皮细胞和平滑肌细胞形态;用DNA图像分析法和生化方法分别测定平滑肌细胞DNA含量和动脉壁胶原纤维含量。结果在ACO造型后7天，钙总量测定显示动脉钙含量较正常大鼠增高约15倍，vonKossa染色证实动脉壁有大量钙沉积，电子探针微分析显示平滑肌细胞内钙积聚。扫描电镜和透射电镜观察显示主动脉内皮细胞早期即有严重损伤。ACO后期出现明显的平滑肌细胞增殖、基质成分改变、血管弹性破坏，表现为动脉壁平滑肌细胞DNA平均含量明显上升，进入增殖周期的细胞数目增多，动脉胶原纤维含量逐步升高。动脉功能测定显示弹性下降，僵硬度增高。结论本方法成功诱导大鼠动脉钙超负荷，且该模型在多方面与人类动脉硬化的特点具有相似性。Objective To induce arterial calcium overload (ACO) in rats and analyze its features.Methods Arterial calcium content and inrtacellular calcium content were determined by atomic absorption spectrometer and electron probe microanalysis, respectiovely . The morphological changes endothelial cells and vascular smooth muscle cells were studied with scanning and transmission electron microscopes. DNA contents and its mass distribution were deterrined by DNA imaging analysis. Arterial collagen and elastin conterts were determimed biochemically . Results Seven days after the induction of ACO with selectal regimen, massive calcium was found accumulating in the arterial wall, including the cytoplasm and mitochondrinon of the medial smooth muscle cells, indicating both extracellular and intlacellular calcium overload Degeneration of aotrtic endothelical cells was found at the early stage of ACO under scaning and transmission electron microscope. At the late time of ACO, significant proliferation and sclerotic reactions of arteries arteries, including the proliferatin of smooth muscle cells, matrix remodeling, and damage of elastic structure. The average DNA content per smooth muscle cell was elevated, and more cells entered the S phase of proliferating cycle since day 7. On the other hand, total collagen content of aorta was increased 7 days ther induction of ACO, and rose continuously at day 14 (P＜0.05) Functional studies showed that arterial elasticity was singificantly impaired. Conclusion Our method could induce ACO constantly in rats, and the ACO model shares the similarities with the chacterization of human arteriosclerosis on many aspects.

关 键 词：钙动脉 血管内皮 动脉硬化 

分 类 号：R543.5[医药卫生—心血管疾病]