p120连环蛋白表达变化对NF-κB信号通路的影响及有关机制  

Effect of p120ctn on NF-κB Signaling Pathway in Human Bronchial Epithelial Cells

作　　者：李丹[1] 夏传生[2] 李娜萍[1] 王曦[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院病理研究所,基础医学院病理学系,武汉430030 [2]中国人民解放军第457医院普外科,武汉430012

出　　处：《华中科技大学学报（医学版）》2011年第3期291-295,共5页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金资助项目(No.81070009)

摘　　要：目的通过甲醛(FA)刺激建立体外气道炎症反应模型,探讨甲醛刺激后人气道上皮细胞株(16HBE)中p120连环蛋白(p120ctn)的表达变化及其对核转录因子-κB(NF-κB)信号通路的影响,从而进一步分析气道炎症发生发展的有关机制。方法培养的16HBE细胞分为对照组和实验组,实验组用100μmol/L的甲醛处理。采用Western blot技术检测甲醛处理不同时间后16HBE细胞p120ctn、NF-κB p65、NF-κB抑制蛋白(IκBα)等蛋白的表达;定量酶联免疫吸附试验(ELISA)检测甲醛处理不同时间后16HBE细胞中白细胞介素8(IL-8)的表达,并与对照组进行比较。结果①p120ctn在正常16HBE细胞中含量丰富,甲醛刺激后,p120ctn表达下调,并呈时间依赖性。②甲醛刺激后引起16HBE细胞中NF-κB p65总蛋白表达量呈时间依赖性增加。③ELISA检测显示甲醛刺激后NF-κB靶基因IL-8表达明显增强。④甲醛刺激后NF-κB抑制蛋白IκBα表达减少;核内NF-κB p65表达增多。⑤甲醛刺激后p120ctn的表达与NF-κB的表达呈负相关。结论在甲醛引起的气道炎症反应中,p120ctn可能通过IκBα降解、释放NF-κB p65入核及促进IL-8表达而负性调节NF-κB信号途径的活化,从而抑制气道炎症反应。Objective To establish an airway inflammation model after formaldehyde（FA）exposure in vitro,detect the changes of p120ctn expression and the effects of p120ctn on NF-κB signaling pathway in 16HBE cells by FA,and analyze the mechanisms of airway inflammation development.Methods 16HBE cells were divided into the control group and the experimental group.They were incubated with FA in the experimental group.The expression levels of p120ctn,NF-κB and IκBα were detected by using Western blot and those of IL-8 by enzyme-linked immunosorbent assay（ELISA）,respectively.Results ①p120ctn was rich in 16HBE cells,but reduced by FA in a time-dependent manner;②The expression of NF-κB p65 was increased in 16HBE cells in a time-dependent manner after FA treatment;③The expression of IL-8 was also increased significantly after FA treatment;④IκBα was rapidly reduced by FA.The expression of p65 in the nucleus was increased after FA treatment;⑤The expression of p120ctn was negatively correlated with that of NF-κB after FA treatment.Conclusion The translocation of p65 into the nucleus is accompanied with IκBα degradation and IL-8 production in 16HBE cells.In the airway inflammation induced by FA,p120ctn may inhibit airway inflammation through regulating NF-κB pathway activation negatively.

关 键 词：P120连环蛋白 NF-ΚB 白细胞介素8 炎症反应 气道上皮细胞 

分 类 号：R562.1[医药卫生—呼吸系统]