Lipo-PGE_1治疗家兔肠系膜静脉血栓模型的实验研究  

Treatment of Mesenteric Venous Thrombosis with Lipo-PGE_1 in a Rabbit Model

作　　者：阳军[1] 索质军[1] 张志伟[1] 郭晖[2] 

机构地区：[1]华中科技大学同济医学院附属同济医院外科,武汉430030 [2]华中科技大学同济医学院附属同济医院器官移植研究所,武汉430030

出　　处：《华中科技大学学报（医学版）》2011年第3期296-300,共5页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

摘　　要：目的探讨脂质微球化前列腺素E1（Lipo-PGE1）治疗家兔肠系膜静脉血栓（MVT）的可行性。方法 30只家兔随机平均分为3组,A、B两组建立MVT模型。A组（模型治疗组）采取帕肝素＋Lipo-PGE1治疗,B组（模型对照组）单用帕肝素治疗,C组（非模型空白对照组）给予帕肝素治疗。分别于5个时间点,建模手术前（T1）、血栓形成后1 h（T2）,以及干预治疗后2 h（T3）4、h（T4）6、h（T5）,检测周围静脉血D-二聚体（DD）值。在实验终点观察小肠黏膜病理改变,计算改良Chiu’s评分。并计算MVT溶解评分和小肠组织含水质量指数。结果在T3~T5时间点,3组血浆DD值差异有统计学意义（P〈0.01）。其中A组DD值分别为（247.5±10.5）,（259.1±9.6）,（273.6±11.9）ng/mL;B组为（231.9±14.1）,（228.7±12.4）,（224.6±12.6）ng/mL;A组高于B组。A、B两组小肠黏膜改良Chiu’s评分分别为（2.00±1.15）vs（4.50±1.08）（P〈0.01）,MVT溶解评分为（2.70±1.25）vs（4.40±0.97）（P〈0.01）。3组小肠组织含水质量指数分别为（73.52±2.40）,（83.90±2.06）,（70.98±1.93）（P〈0.01）。A组病理改变均优于B组。结论 Lipo-PGE1可增强家兔MVT模型体内纤溶活性,减轻受累小肠病理损害,促进MVT血栓溶解,对治疗MVT具有潜在价值。Objective To investigate the possibility of using Lipo-PGE1 to treat acute mesenteric vein thrombosis（MVT）in a rabbit model.Methods Thirty rabbits were equally divided into three groups.Rabbit MVT models were established in group A and group B.In group A,the rabbits were treated with parnaparin＋Lipo-PGE1,and those in group B only with parnaparin.The rabbits in group C（control group of no-MVT-model）were treated with parnaparin.The D-dimer levels from the peripheral vein were measured at five time points：before surgery,1 h after MVT,and 2,4,6 h after the treatment,named T1-T5 respectively.The pathologic changes in mucosa of small intestine were observed at the end of the experiment and the scores according to the Chiu-Park Scale were calculated.At the same time the MVT-thrombolysis Scale and the index of hydrated quality of the small intestine were calculated too.Results At the time points of T3 to T5,there was significant difference in the D-dimer levels among groups A,B,C（P〈0.01）.At T3 to T5,the D-dimer levels in group A were（247.5±10.5）,（259.1±9.6） and（273.6±11.9） ng/mL separately,which were higher than in group B[（231.9±14.1）,（228.7±12.4）,（224.6±12.6） ng/mL separately].At the end of the experiment,the scores of Chiu-Park Scale in groups A and group B were（2.00±1.15） and（4.50±1.08） respectively（P〈0.01）.The scores of the MVT-thrombolysis Scale in groups A and B were（2.70±1.25） and（4.40±0.97） respectively（P〈0.01）.The indexes of hydrated quality of the small intestine in groups A,B,C were（73.52±2.40）,（83.90±2.06） and（70.98±1.93） respectively（P〈0.01）.The pathologic changes in group A were milder than in group B.Conclusion In the rabbit MVT models,Lipo-PGE1 could enhance the thrombolysis activity,alleviate the pathologic lesions of the suffered small intestine and accelerate thrombolysis of MVT.

关 键 词：肠系膜静脉血栓 前列腺素E1 D-二聚体 

分 类 号：R453.9[医药卫生—治疗学]