小单孢菌211003的分离及16S rRNA基因序列分析  

Isolation and 16S rRNA Gene Sequence Analysis of Micromonospora sp.211003

作　　者：徐小雄[1,2] 林海鹏 谢晴宜 洪葵 

机构地区：[1]琼州学院,海南三亚572022 [2]海南省两栖爬行动物重点实验室(筹) [3]热带生物技术研究所

出　　处：《现代农业科技》2011年第11期47-48,共2页Modern Agricultural Science and Technology

摘　　要：小单孢菌211003分离自海南红树植物海膝根围土壤,采用干热100℃、60 min的预处理,葡萄糖天门冬素培养基(GA)作为分离培养基。该菌株革兰氏阳性,橘黄色基内菌丝,产单个孢子。经16S rRNA基因序列分析,归到小单孢菌属,并成一独立分支。并与M.siamensis TT2-4T的相似率最高,为98.415%。初步判定菌株211003为小单孢菌属一个潜在的新种。Using pretreatment of hot heat 100 ℃ for 60 min,Micromonspora sp.211003 was isolated from GA-vitamin selective media.The strain is G＋,and single spores were formed on yellowish substrate hyphae.An almost-complete 16S rRNA gene sequence analysis showed the isolate was assigned to the genus Micromonospora and was form a cluster separately.The highest levels of similarity were with M.siamensis TT2-4T（98.415%）.Based on the analysis,the isolate should be a new member of Micromonospora.

关 键 词：小单孢菌 分离 16S RRNA基因序列 

分 类 号：Q939.139[生物学—微生物学] Q78