UVB照射对PIG1细胞增殖活性的影响  

Effect of UVB irradiation on the proliferation of PIG1 cells

作　　者：朱东宁[1] 简强[1] 薛柯[1] 刘博强[1] 王玲[1] 李承新[1] 

机构地区：[1]第四军医大学西京医院皮肤科,陕西西安710032

出　　处：《中国美容医学》2011年第6期944-946,共3页Chinese Journal of Aesthetic Medicine

摘　　要：目的:探讨UVB照射对正常人黑素细胞PIG1细胞形态及增殖活性的影响。方法:取对数生长期的PIG1细胞,分别以50、100、200、300、400、500、600和700mJ/cm2剂量的UVB照射后继续培养0h、24h和48h,在倒置显微镜下观察其形态学变化,用MTT法检测UVB照射对细胞增殖活性的影响。结果:在50～200 mJ/cm2的UVB辐射下,随辐射剂量的增大,PIG1细胞的增殖活性逐渐增加,倒置相差显微镜从形态学上证实了一定剂量的UVB照射可以促进细胞增殖。结论:一定剂量的UVB照射有刺激PIG1细胞增殖及树突生长的作用。Objective To investigate the effect of UVB irradiation on the proliferation of PIG1 cells.Methods Morphology change of PIG1 cells was observed by light microscopy.MTT assay was used to record cellular activity.Results After UVB irradiation,the number of PIG1 cells was increased and the length of dendrites was extended.The proliferation activity of PIG1 cells was dependent on the irradiated dosages（50～200mJ/cm2）.Conclusions UVB irradiation can promote the proliferation activity of PIG1 cells and extend the dendrites of PIG1.

关 键 词：PIG1细胞 UVB照射 增殖 树突 

分 类 号：Q813.1[生物学—生物工程]