辣椒小孢子母细胞减数分裂过程中微管的分布变化  

Microtubule Distribution and Change During Meiosis of Microspore Mother Cells of Capsicum Annum L.

作　　者：刘胜姿[1] 王留英[2] 邱义兰[2] 刘如石[2] 王晓辉[2] 刘少军[2] 

机构地区：[1]湖南师范大学医学院,中国长沙410081 [2]湖南师范大学生命科学学院,中国长沙410081

出　　处：《湖南师范大学自然科学学报》2011年第3期65-68,共4页Journal of Natural Science of Hunan Normal University

基　　金：中国博士后科学基金一般资助项目(20080440983)中国博士后科学基金特别资助项目(201003513);湖南省科技计划博士后专项资助项目(2009RS3013)

摘　　要：以辣椒牛角椒2号为材料,应用间接免疫荧光标记技术观察了小孢子母细胞减数分裂过程中微管分布变化.结果表明,减数分裂之前,小孢子母细胞中的微管较短,分散在细胞质中.在减数分裂I中期,细胞质中的微管形成纺锤体.进入减数分裂Ⅰ后期,纺锤体微管缩短,被拉向细胞两极的染色体之间存在极丝微管.到减数分裂Ⅰ末期,微管集中在2个新形成子核的表面.进入减数分裂II,细胞中的微管又集中形成2个纺锤体,随后2个纺锤体微管缩短.进入减数分裂II末期,在4个新形成的子核之间存在长又直的微管,最后微管解聚为微管蛋白分散在细胞质中.辣椒胞质分裂发生在4个细胞核形成之后,通过细胞核之间的质膜向内缢缩分割4个细胞核,产生4个小孢子.By using the technique of indirect immunofluorescence labeling,the distribution of microtubule（MT） cytoskeleton during meiosis of microspore mother cells of Capsicum annum L.is observed.Before meiosis of microspore mother cell（MMC）,MTs in the cells are short and dispersive in its cytoplasm.At metaphase I,MTs in the cytoplasm set up a spindle.When MMCs enter anaphase I,the spindle MTs are shortened and being polar MTs between bipolar chromosomes.At the telophase I,MTs are mostly concentrated on the nucleate surface of two nuclei.As meiosis II began,MTs in cytoplasm form two spindles at metaphase II.Then,the two spindle MTs shorten.When entering telophase II,MMCs form four nucleus and being long and straight MTs between each two nuclei.The MMCs began to cytokinesis by cytoplasmic constriction after four nucleus formed.At last,MTs are radiated from the surface of the nucleus to the surrounding cytoplasm in the cytoplasm of tetrad microspores.

关 键 词：辣椒 小孢子 减数分裂 微管 

分 类 号：Q245[生物学—细胞生物学]