Gel-HA-M/PRP复合材料对种植体周围骨缺损修复的实验研究  

The Experimental Research in Repairing the Bone Defect Around Dental Implant by a New Type Material

作　　者：尚新华[1] 周健[1] 李全利[1] 孙传锋[1] 窦晓晨[1] 

机构地区：[1]安徽医科大学口腔医学院.附属口腔医院,安徽合肥230032

出　　处：《口腔颌面外科杂志》2011年第3期167-171,共5页Journal of Oral and Maxillofacial Surgery

基　　金：国家自然科学基金资助项目(307724400);安徽省科技攻关重点项目(08010302196);安徽医科大学校科研基金资助项目(2011xkj017)

摘　　要：目的:观察明胶-羟基磷灰石-米诺环素(Gel-HA-M)纳米复合物与富血小板血浆(platelet-rich plasma,PRP)复合(GEL-HA-M/PRP)修复种植体周围骨缺损的效果,探讨其作为种植体周围骨缺损修复材料的可行性。方法:普通健康杂种犬6只,拔除双侧下颌第二、三、四前磨牙,3个月后每侧拔牙处植入3枚直径4 mm、长11 mm的种植体,并于钛钉周围制造宽约1.5 mm、深4 mm的环形骨缺损,分别植入自体骨、Gel-HA-M/PRP,最后为空白作为对照。种植术后4、8、12周各处死2只动物,进行大体、影像学及组织学观察。结果:实验组种植体周围骨缺损区较空白对照组新骨形成量多、外形好,并未见材料剩余;自体骨组可见成熟骨组织,较多新骨形成,同时有少量骨吸收存在;空白组主要为纤维性结合,有少量的骨组织再生。结论:提示Gel-HA-M纳米复合物的PRP复合凝胶能够促进种植体周围骨缺损的修复。Objective： To investigate the feasibility of a hybrid biomaterial gelatin-apatite-minocycline nanocomposite combined with platelet-rich plasma（Gel-HA-M/PRP） to repair the bone defects around dental implants.Methods： 6 healthy mongrel dogs,their 2nd,3rd,and 4th premolars were extracted from both sides of the mandible.Three months after extraction,3 implants with the diameter of 4 mm and length of 11 mm were separately implanted in each side of the mandible.At the same time,a bone defect with the size of 1.5 mm in width and 4 mm in depth was created around the neck of each implant.The bone defects were grafted with autogenous bone,Gel-HA-M/PRP,and kept void as control respectively.Every 2 dogs were sacrificed at 4-,8-,and 12-week respectively after the implantation.Specimen were examined by X-ray and histopathology sections.Results： In hybrid material grafted wounds,more new bone formation were observed,the Gel-HA-M nanocomposite was fully adsorbed.In autogenous bone grafted wounds,mature bone and a few new bone formation were revealed and also bone resorption appeared.In blank control wounds,fibrous connective tissue almost filled the bone defect,and only a small amount of bone tissue regenerated.Conclusion： The hybrid combination of gelatin-apitate-minocycline nanocomposite plus platelet-rich-plasma can effectively repair bone defect around implants.

关 键 词：明胶 羟基磷灰石 米诺环素 种植体 骨缺损 富血小板血浆 

分 类 号：R318.08[医药卫生—生物医学工程] R782.13[医药卫生—基础医学]