共刺激分子OX40-OX40L相互作用对ApoE-/-小鼠淋巴细胞活化T细胞核因子C1表达的影响  

Effect of the costimulatory molecules OX40-OX40 ligand interaction on the expression of NFATc1 in leukocytes of apolipoprotein E-deficient mice

作　　者：徐良洁[1] 严金川[1] 王标[1] 刘培晶[1] 龚杰[1] 王翠平[1] 

机构地区：[1]江苏大学附属医院心内科,镇江212001

出　　处：《中华心血管病杂志》2011年第6期526-530,共5页Chinese Journal of Cardiology

基　　金：江苏省医学重点人才基金（RC2007033）;镇江市社会发展项目（SH2007020）

摘　　要：目的 探讨共刺激分子OX40-OX40L相互作用对载脂蛋白E基因敲除（ApoE-/-）小鼠淋巴细胞中活化T细胞核因子C1（NFATc1）表达的影响.方法 采用未经治疗的ApoE-/-小鼠颈动脉粥样斑块模型,取其脾脏淋巴细胞,以不同浓度刺激型OX40抗体或抑制型OX40L抗体体外特异性刺激或阻断OX40-OX40L相互作用,不同时间点收获细胞,分别应用实时荧光定量PCR和流式细胞技术检测小鼠淋巴细胞NFATc1 mRNA和蛋白表达.结果 （1）抗OX40抗体特异性刺激OX40-OX40L轴后,小鼠淋巴细胞NFATc1 mRNA及蛋白表达明显上调,anti-OX40浓度为20 μg/ml时刺激作用最强,刺激时间24 h最佳（P〈0.01）.（2）抗OX40L抗体特异性阻断OX40-OX40L轴能明显抑制NFATc1 mRNA及蛋白表达,anti-OX40L为20 μg/ml时抑制作用最强,抑制时间24 h最佳（P〈0.001）.结论 OX40-OX40L相互作用能调控ApoE-/-小鼠淋巴细胞NFATc1的表达.Objective To investigate the effect of OX40/OX40L interaction on the nuclear factor of activated T cells c1（NFATc1） in ApoE-/- mice.Methods Lymphocytes were prepared from mouse spleens after Collar-treated Surgery, then incubated with a range of agonistic anti-OX40 mAbs and inhibitory anti-OX40L mAb to stimulate or inhibit OX40-OX40L interaction in vitro. The expression of NFATc1 mRNA and protein in lymphocytes of ApoE-/- mice was measured by Real Time PCR and flow cytometry, respectively.Results （1）After stimulating OX40-OX40L signal pathway, the expression of NFATc1 mRNA and protein in leukocytes of ApoE-/- mice was significantly increased, with maximal effect occurring at 20 μg/ml anti-OX40 mAb- stimulated, and peaked at 24 h at any concentration（P〈0.01）. （2）Anti-OX40L mAb significantly suppressed the expression of NFATc1 in leukocytes of ApoE-/- mice, with maximal effect occurring at 20 μg/ml anti-OX40L mAb, and peaked at 24 h（P〈0.001）.ConclusionsOX40-OX40L interaction can regulate the mRNA and protein expressions of NFATc1 in lymphocytes of ApoE-/- mice.

关 键 词：动脉粥样硬化 OX40 OX40配体 NFATC转录因子类 

分 类 号：R543.5[医药卫生—心血管疾病]