多壁碳纳米管对单核巨噬细胞的毒性作用  

Toxic effect of multi-walled carbon nanotubes on macrophages

作　　者：罗蔓[1] 邓小勇[2] 董玲[1] 沈锡中[1] 

机构地区：[1]复旦大学附属中山医院,上海200032 [2]上海大学纳米化学与生物学研究所,上海200444

出　　处：《解剖学杂志》2011年第3期319-323,共5页Chinese Journal of Anatomy

摘　　要：目的：研究多壁碳纳米管（p-MWCNTs）与牛磺酸修饰的多壁碳钠米管（tau-MWCNTs）在体外对小鼠的单核巨噬细胞RAW264．7细胞的毒性作用。方法：p-MWCNTs与tau-MWCNTs作用于RAW264．7细胞后，观察RAW264．7细胞乳酸脱氢酶（LDH）释放率、细胞形态、肿瘤坏死因子a（TNF-a）和凋亡情况。结果：P-MWCNTs可以使细胞的LDH释放率增高，诱发细胞发生凋亡，增加细胞内TNF-a释放，而即使高浓度的tau-MWCNTs对RAW264．7细胞也没有毒性作用。结论：tau-MWCNTs不影响细胞活力，它可以在生物与医学方面得到广泛的应用，To study the toxicological effects and mechanisms of the p-MWCNTs and tau-MWCNTs on macrophages in vitro. Methods： We focused on their effects on ultrastructural changes in cell morphology, LDH release, the release of tumour necrosis factor （TNF）-a, as well as annexin V/PI staining. Results： Exposure of RAW264. 7 macrophages to pMWCNTs at a high dosage induced cellular apoptosis, and an increase in LDH release and TNF-a. Whereas tau-MWCNTs had only very limited impact on cellular toxicity and apoptosis. Conclusion： Our results imply that the tau-MWCNTs should be used for biological or medical purposes without affecting the cell viability.

关 键 词：多壁碳纳米管 RAW264.7细胞 肿瘤坏死因子A 凋亡 

分 类 号：R285.5[医药卫生—中药学]