生后大鼠海马CA区CXCR4蛋白的表达变化  

Expressional changes of CXCR4 proteins in the postnatal rat hippocampal CA area

作　　者：王玉兰[1] 徐红[1] 李爱娜[1] 

机构地区：[1]徐州医学院解剖学教研室,徐州221002

出　　处：《解剖学杂志》2011年第3期336-338,共3页Chinese Journal of Anatomy

基　　金：江苏省高校自然科学研究计划（08KJB310013）;徐州医学院院长专项人才基金（08KJZ03）.

摘　　要：目的：研究CXCR4蛋白在生后大鼠海马CA区的表达变化。方法：用免疫印迹法和免疫组织化学法定量、定位检测CXCR4在海马CA区的表达变化。结果：从生后4d到成年大鼠海马CA区均可见CXCR4蛋白表达阳性的细胞，其胞质呈棕黄色。生后早期，可见CA区锥体细胞伸出的轴丘上有棕黄色显色；后期其长轴突及短树突上也有不同程度CXCR4蛋白表达。免疫印迹检测显示，生后各时间点在分子量为43kD处均检测到CXCR4阳性条带。CXCR4在CA区的相对表达量于生后1周达高峰，随年龄增长逐渐下降，至生后3周CXCR4蛋白表达降至成年水平。结论：CXCR4蛋白在大鼠海马CA区的表达具有时空性差异，提示CXCR4参与了大鼠海马生后发育过程，并可能与不同时期海马结构和功能的发育有关。XTo investigate expressional changes of CXCR4 proteins in CA area during the development of the rat hippocampus. Methods： Western blot and immunohistochemistry analyses were used to detect CXCR4 proteins expression in the CA area of the hippocampus at different age. Results： The pyramidal cells expressed CXCR4 proteins in the CA field of the rat hippocampus from postnatal 4 day to adult, and its cytoplasm was stained. The axon hillock of pyramidal cells was stained in CA area at postnatal prophase; and long axon and short dendrites were stained at the postnatal later stag~ Western blot results showed that the expression of CXCR4 proteins reached its peak level at postnatal 1 week. After that, it decreased gradually and fell to adult level at postnatal 3 week. Conclusion： The CXCR4 expressions are spatial and temporally different in the CA area of the postnatal rat hippocampus. This implies that CXCR4 play a role during the rat hippocampal development, and may be concerned with structural and functional alterations of the hippocampus in different periods of development.

关 键 词：生后 海马 CA区 CXCR4 大鼠 

分 类 号：R512.91[医药卫生—内科学]