人微小病毒B19致胎肝造血细胞凋亡及其基因表达的意义  

Significance of apoptosis and its gene expression in hematopoietic cells of fetal liver induced by human parvovirus B19

作　　者：孙新[1] 张国成[1] 韩美玉[1] 许东亮[1] 李飚[1] 

机构地区：[1]第四军医大学西京医院儿科,陕西西安710033

出　　处：《第四军医大学学报》1999年第9期750-752,共3页Journal of the Fourth Military Medical University

基　　金：国家计生委基金

摘　　要：目的：研究人微小病毒 Ｂ１９（ Ｈ Ｐ Ｖ Ｂ１９）引致胎肝造血细胞凋亡及凋亡相关基因表达的变化，初步探讨 Ｈ Ｐ Ｖ Ｂ１９ 引起死胎的机理． 方法：采用原位末端标记法对２５ 例石蜡包埋死胎肝脏组织（其中 Ｈ Ｐ Ｖ Ｂ１９ Ｄ Ｎ Ａ 阳性者 １０ 例， Ｈ Ｐ Ｖ Ｂ１９ Ｄ Ｎ Ａ 阴性者１５ 例）进行细胞凋亡检测，并用免疫组化方法检测ｂｃ１２，ｂａｘ 表达情况． 结果：１０ 例 Ｈ Ｐ Ｖ Ｂ１９ Ｄ Ｎ Ａ 阳性死胎肝脏组织中 ８ 例明显可见造血细胞的凋亡，６ 例 ｂａｘ 表达阳性． １５ 例 Ｈ Ｐ Ｖ Ｂ１９ Ｄ Ｎ Ａ 阴性死胎肝脏组织中仅 ２ 例有造血细胞的凋亡，２ 例ｂａｘ 表达阳性，２５ 例均未检出ｂｃ１２ 的表达．结论： Ｂ１９ 感染可能诱导胎肝造血细胞的凋亡，这为可能是该病毒引起胎儿死亡的重要机制．AIM:To investigate the apoptosis and its gene expression in hematopoietic cells of fetal liver. METHODS: In situ end labeling (ISEL) was used to detect the apoptosis in 25 paraffin embedded fetal liver tissue samples and immunohistochemical method was used to investigate the expression of the bcl 2 and bax gene. RESULTS: Apoptosis was observed in 8 HPVB19 DNA positive cases (8/10), and the expression of bax was present in 6 cases(6/10). Apoptosis was only found in 2 HPVB19 DNA negative cases and the expression of bax was also present in 2 cases. But the expression of bcl 2 was found in none of the 25 cases. CONCLUSION: HPVB19 may induce apoptosis of hematopoietic cells in fetal liver and the virus may cause fetal death by this way.

关 键 词：微小病毒B19 细胞凋亡 基因表达 胎儿 

分 类 号：R373[医药卫生—病原生物学] R329.2[医药卫生—基础医学]