小鼠转轮运动昼夜节律研究技术的建立  

Techniques for Measuring Mice Circadian Wheel Running Activities.

作　　者：周岚[1] 曹济民[1] 

机构地区：[1]中国医学科学院基础医学研究所/北京协和医学院基础学院生理和病理生理学系,北京100005

出　　处：《医学研究杂志》2011年第6期31-35,共5页Journal of Medical Research

基　　金：国家自然科学基金资助项目(81071072);科技部"973"课题(2011CB933504)

摘　　要：目的建立一种实时监测小鼠昼夜运动节律的技术。方法将转轮装置及配套设备进行安装和调试，然后实时连续记录C57BL／6J小鼠的转轮运动，并通过计算小鼠转轮运动周期、诱导后相角、周期幅度以及运动活性等指标来分析小鼠的昼夜运动节律。结果运用转轮装置可以准确地记录到小鼠昼夜运动节律。C57BL／6J小鼠诱导后相角为（145°～170°）±6°，在持续黑暗（DD）环境下运动昼夜节律为23．54±0．3h。结果还发现，这种昼夜运动活性在小鼠间有一定个体差异。分别在CT（circadian time）14和CT22给予15min光诱导，能引起小鼠运动节律分别向后及向前漂移。结论这种转轮装置及分析方法是一种可靠、有效的研究小鼠昼夜运动节律的技术平台。Abstract Objective To establish the techniques for recording the circadian wheel running activities in mice. Methods We gave an outline of the system setup and the factors necessary to measure mouse circadian rhythms, including choice of mouse strain, facilities and equipment design and construction, experimental design, high - throughput methods, and finally methods for data analysis. Results The phase angle of entrainment of C57BL/6J mice was （ 145±170°） ±6° and the free running period was 23.5±0.3h. The average daily activity was calculated by the sum of the number of wheel revolutions divided by the total numbers of days. The light （100Lux） entrainment at CT14 and CT22 could reset the circadian rhythm. When applied at CT14, light exposure induced a phase - delay of the circadian rhythm of locomotor activity and a phase - advance at CT22. Conclusion The established circadian wheel running setup provides an sensitive and quantitative method for circadian rhythm study.

关 键 词：转轮运动 转轮装置 昼夜节律 生物钟 小鼠 

分 类 号：R587.102[医药卫生—内分泌]