TLR2和TLR4在原因不明复发性流产绒毛和蜕膜中的表达及其意义  

Expression of TLR2 and TLR4 on Decidual and Villi in Unexplained Recurrent Spontaneous Abortion and Its Significance.

作　　者：翟洪波[1] 徐键[1] 

机构地区：[1]浙江大学附属妇产科医院,杭州310006

出　　处：《医学研究杂志》2011年第6期80-83,共4页Journal of Medical Research

基　　金：浙江省自然科学基金资助项目(Y2080397)

摘　　要：目的本实验通过检测原因不明复发性流产（URSA）患者和健康早孕者TLR2和TLR4在子宫绒毛和蜕膜中的表达，及外周血清中TNF-α及IL-10的水平，初步探讨TLR2和TLR4与URSA存在相关性，以及在URSA发生发展中的可能途径。方法前瞻性研究2008～2009年在浙江大学医学院附属妇产科医院和杭州市第一人民医院门诊患者。年龄在25～35周岁，孕龄〈12周。将有≥3次自然流产的URSA患者作为研究组；将曾有正常生育史，本次要求行人工流产终止妊娠的早孕健康妇女作为对照组，分别选择20例。免疫组化检测TLR2和TLR4在两组绒毛和蜕膜中的表达；用ELISA法检测血清TNF—α及IL-10的水平，比较是否存在差异性。结果①TLR2在两组绒毛、蜕膜中表达差异无统计学意义（P〉0．05）；②TLR4在研究组绒毛、蜕膜表达明显增强，有显著统计学意义（P〈0．05）；③TNF—d在研究组中的水平明显增高，有显著统计学意义（P〈0．05）；IL—10在两组中的差异无统计学意义（P〉0．05）。结论TLR4可能是通过释放过量的Th1型细胞因子，导致Th1／Th2失衡，从而极可能参与URSA的发病。Objective In this study, by detecting the TLR2, TLR4 in the expression of villi and decidua of unexplained recurrent spontaneous abortion （URSA） and early pregnancy, we can preliminarily study the role of TLR2 and TLR4 in the genesis and development of URSA. Meantime, by detecting serum level of TNF - et and IL - 10, we can preliminarily learn the available ways of TLR2 and TLR4 in the occurrence of URSA. Methods Prospective study on outpatients of Obstetrics and Gynecology at the Medical College of Zhejiang U- niversity and Hangzhou First People＇s Hospitalin 2008 -2009 was carried out. Those women whose age were between 25 -35 years old, and gestational age 〈 12 weeks were put together as a study group. For those URSA patients who have been spontaneously aborted for ≥3 times, we regarded then as study group. While for those healthy patients who had normal reproductive history,and demanded induced abortion in early pregnancy termination of pregnancy,we distinguished them as a control group. We selected both URSA patients （study group） and healthy pregnant patients （control group） with 20 cases. By immunohistoehemical staining, we detected the expression of TLR2 and TLR4 in villi and decidua to compare the different expression of TLR2 and TLR4 in villi and deeidua of URSA and early preg- nancy. At the same time, we detected serum concentration of TNF -ct and IL- 10 with ELISA to compare the concentration differences of serum TNF - ct and IL - 10 between URSA and early pregnancy. Results ①No significant difference in expression of TLR2 in the cyto- plasm and the membrane of villous trophoblast ceils and glandular epithelial cells was found between two groups （ P 〉 0.05 ）. ②Compared with contrast group, expression of TLR4 in the cytoplasm and the membrane of trophoblast cells and glandular epithelial cells in study group was significantly increased,and the differences were statistically significant （P 〈 0.05）. （~）Compared with the control group, con- centration of TNF -ct in the

关 键 词：不明原因复发性流产 TLR2 TLR4 

分 类 号：R714.21[医药卫生—妇产科学]