基于银纳米粒子构建荧光传感平台用于核酸检测  

Silver Nanoparticles as a Fluorescent Sensing Platform for Nucleic Acid Detection

作　　者：张瑛洧[1] 李海龙[1,2] 孙旭平[1] 

机构地区：[1]中国科学院长春应用化学研究所电分析国家重点实验室,长春130012 [2]中国科学院研究生院,北京100039

出　　处：《分析化学》2011年第7期998-1002,共5页Chinese Journal of Analytical Chemistry

基　　金：中国国家重点基础研究发展计划(No.2011CB935800)资助

摘　　要：报道了基于银纳米粒子构建的荧光传感平台,并用于核酸检测。此荧光传感平台对核酸检测基于以下策略:首先,荧光团标记的单链DNA探针被吸附到银纳米粒子的表面,荧光团与银纳米粒子近距离接触,发生荧光猝灭;加入与探针DNA序列互补的目标DNA,两者杂交形成双链DNA,并从银纳米粒子的表面脱离,荧光得到恢复。这种银纳米粒子构建的荧光传感平台对于完全互补和碱基错配的DNA序列具有良好的区分能力。In this study,we reported Ag nanoparticles（AgNPs） as an effective fluorescent sensing platform for nucleic acid detection for the first time.The general concept used in this approach is based on adsorption of the fluorescently labeled single-stranded DNA（ssDNA） probe by AgNP,which is accompanied by substantial fluorescence quenching due to their close proximity,followed by specific hybridization with its target to form a double-stranded DNA（dsDNA）.The use of this sensing platform to differentiate complementary and mismatched sequences is also demonstrated.

关 键 词：核酸检测 荧光传感平台 银纳米粒子 

分 类 号：Q52[生物学—生物化学]