高灵敏、易更新的土霉素分子印迹碳糊传感器的研制  

A Strategy for Constructing Sensitive and Renewable Molecular Imprinted Carbon Paste Sensor

作　　者：陈志强[1] 李建平[1] 李玉平[1] 

机构地区：[1]桂林理工大学化学与生物工程学院,桂林541004

出　　处：《分析化学》2011年第7期1009-1014,共6页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.20665003);广西自然科学基金(No.0728214)资助

摘　　要：通过将土霉素(OTC)分子印迹聚合物颗粒与碳糊混合,制成OTC分子印迹固体石蜡碳糊传感器(OTC-MICPS)。在分子印迹膜(MIP)上,OTC与辣根过氧化物酶(HRP)标记的OTC(HRP-OTC)之间进行竞争取代反应,标记的HRP可催化H2O2氧化对苯二酚生成对苯二醌,通过测量对苯二醌的还原电流间接测定OTC。对实验条件进行了优化,响应电流与OTC浓度(0.008～0.6μmol/L)的对数呈线性关系,检出限为1.8 nmol/L。此传感器可以通过简单的机械抛光实现更新。传感器制作简单,使用方便,价格低廉,灵敏度高,易于更新。用于牛奶样品的分析测定,加标回收率为93.8%～96.6%。Oxytetracycline（OTC） molecular imprinted particles and carbon paste were mixed to prepare OTC molecular imprinted carbon paste sensor.The sensor could specifically recognize OTC and horseradish peroxidase（HRP） labeled OTC by the competition replacement reaction between them.The labeled HRP catalyzed the reaction between H2O2 and mediator hydroquinone,and the detection of OTC was based on the voltammetric monitoring of the electroreduction of the oxidized mediator,benzoquinone.A linear relationship between response current and logarithm of concentrations of OTC in the concentration range of 0.008-0.6μmol/L was obtained with a detection limit of 1.8 nmol/L.The sensor renewal was achieved by a simple mechanical polishing step of sensors′surface.The sensor is easy to prepare and use,low cost,high sensitivity and easy renewal.The sensor has been successfully applied to the determination of OTC in milk samples.The recoveries were 93.8%-96.6%.

关 键 词：分子印迹 土霉素 电化学传感器 辣根过氧化物酶 碳糊 

分 类 号：O657.1[理学—分析化学] TP212.2[理学—化学]