葡萄糖检测用分子印迹光子晶体的研究  

Molecularly Imprinted Photonic Crystal for Detection of Glucose

作　　者：薛飞[1] 段廷蕊[1] 薛敏[1] 刘烽[1] 王一飞[1] 韦泽全[1] 王淼[1] 张盼[1] 袁梓高[1] 孟子晖[1] 

机构地区：[1]北京理工大学化工与环境学院,北京100081

出　　处：《分析化学》2011年第7期1015-1020,共6页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.20775007);863计划(No.2007AA10Z433);环境毒理国家重点实验室开放基金(No.KF2009-08)资助

摘　　要：基于快速、持续、无创检测技术在血糖监测领域的重要性,以聚甲基丙烯酸甲酯微球阵列为光子晶体制孔模板,以葡萄糖为印迹模板,N-异丙基丙烯酰胺和甲基丙烯酸羟乙酯为混合单体,4-乙烯基苯硼酸为识别基,N,N-亚甲基双丙烯酰胺为交联剂,在制孔模板间隙进行共价型分子印迹聚合,除去制孔模板后,制得具有规整孔结构的新型光学凝胶材料——分子印迹光子晶体。实验表明:此材料对葡萄糖响应速度快,选择性高,印迹聚合物对葡萄糖吸附量在3 min即达到3.2 mg/g。在同等条件下,印迹聚合物对葡萄糖的吸附量是其它类似物吸附量的2倍以上。印迹聚合物的识别行为以衍射光波长变化形式表现,随着葡萄糖浓度由0 mol/L增加到0.02 mol/L,葡萄糖分子印迹光子晶体的衍射波长发生150 nm的红移,表现出良好的传感特性。此材料为葡萄糖检测提供了新思路,有望用于人体泪液或尿液中葡萄糖浓度的检测,实现非侵入式的检测手段。A three-dimensionally ordered,macroporous,inverse-opal glucose molecularly imprinted polymer crystal（MIPC） film was synthesized by the inverted colloidal-crystal template technique.Preparation of our MIPC was carried out by thermo-poymerization of monomers（2-hydroxyethyl methacrylate and N-isopropylacrylamide）,covalent recognizer（4-ethylenyl phenylboronic acid）,and cross-linker（N,N′-Methylenebisacrylamide） with poly（methyl-methacrylate）（PMMA） particle array as template,followed by chemical removal of the template using acetone.Due to its 3D-ordered macroporous arrays with molecular imprinting,nanocavities,the formed photonic hydrogel allows rapid and selective detection of the target glucose.The glucose adsorption amount of our MIP was 3.2 mg/g in 3 min which is two times more than that of other analogues.The diffraction wavelength of the MIPC redshifts was more than 150 nm in response to 0.02 mol/L of glucose.Our results revealed that MIPC could provide a novel non-invasive approach for glucose detection in tears or urine and exhibit a promising potential for development of new glucose monitoring technology.

关 键 词：光子晶体 分子印迹 葡萄糖检测 自组装 

分 类 号：O629.1[理学—有机化学]