肾性高血压大鼠血浆及血管组织中内皮素及基础NO的动态变化  

The Dynamic Changes of ET in Plasma and Aortic Tissue,Basal NO Release from Aortic Endothelium in Renal Hypertensive Rats

作　　者：吴秀香[1] 董建文[1] 贾月霞[1] 时安云[1] 

机构地区：[1]北京医科大学病理生理教研室

出　　处：《高血压杂志》1999年第4期366-367,共2页Chinese Journal of Hypertension

基　　金：国家科委及卫生部基金!资助课题(No. 94- 1- 275)

摘　　要：目的：观察肾性高血压（ＲＨ）时，血浆及血管组织中内皮素（ｅｎｄｏｔｈｅｌｉｎ， ＥＴ）及基础ＮＯ的动态变化，探讨其在肾性高血压发生发展中的作用。方法：将体重为１６０～１８０ ｇ 雄性Ｗｉｓｔａｒ大鼠随机分为肾性高血压组及假手术组，术后饲养２，４，６ 周后测定大鼠血压的变化、血浆及主动脉中ＥＴ 含量、主动脉基础一氧化氮（ｎｉｔｒｉｃｏｘｉｄｅ， ＮＯ）释放量。结果：ＲＨ组术后２周时血压即已升高，４、６ 周时显著升高。ＲＨ组２，４，６周基础ＮＯ生成量显著低于同期Ｃ组，而血浆及主动脉中ＥＴ含量均显著高于同期Ｃ组。结论：肾性高血压时，血管内皮细胞功能紊乱，维持血管正常张力的基础ＮＯ生成减少。Objective:To observe the dynamic change of endothelin(ET) in plasma and aortic tissue, basal NO release from aortic endothelium in renal hypertensive rats orderto explore the roles of them in the initiation and maintenance of renal hypertension. Methods: Male Wistar rats weighing 160～180 g were divided into two groups: renal hypertension (RH) group and control(c) group and fed for 2,4,6 weeks respectively. Blood pressure, basal NO, plasma and aorta ET were measured by inserting a cannula into the carotid artery using bioassay and radioimmunoassay respectively. Results: The blood pressure increased in 2 weeks and markedly increased in 4,6 weeks in RH rats. Basal NO release from endothelial cells of 2,4 6 weeks RH rats was reduced. Meanwhile, the contents of ET in plasma and aortic tissue were markedly increased. Conclusions:The vascular endothelial cells function of renal hypertension rats was disturbed. The balance of vascular regulating system, basal NO (relaxation) and ET (contraction),was impaired in RH rats. This might be involved in the initiation and maintenance of renal hypertension.

关 键 词：肾性高血压 内皮素 一氧化氮 

分 类 号：R544.140.2[医药卫生—心血管疾病]