自发性高血压大鼠冠状动脉平滑肌细胞膜电位的研究  

Membrane Potential of Coronary Arterial Smooth Muscle Cells in \;Spontaneously Hypertensive Rats

作　　者：王福文[1] 刘志向[2] 解砚英[1] 王元书[1] 柴象枢[2] 

机构地区：[1]山东省医学科学院药物所 [2]山东省医学科学院基础所,济南250062

出　　处：《高血压杂志》1999年第4期380-382,共3页Chinese Journal of Hypertension

基　　金：国家自然科学基金!资助项目(No.39239150)

摘　　要：目的：观察自发性高血压大鼠（ＳＨＲ）冠状动脉平滑肌细胞的静息膜电位（Ｅｍ ）及其对血管活性物质ＫＣｌ、ＡＣｈ、ＮＥ的反应性，并与Ｗｉｓｔａｒ大鼠进行了比较。方法：采用先进技术细胞内微电极的方法。结果：ＳＨＲ冠状动脉平滑肌细胞静息膜电位稳定，其均值为－４２．４０±２．７０ ｍ Ｖ，比Ｗｉｓｔａｒ大鼠升高２２％ 左右；ＫＣｌ和ＡＣｈ均引起膜电位去极化，且呈剂量依赖式特点；ＮＥ对膜电位无明显影响。结论；　ＳＨＲ的膜电位比Ｗｉｓｔａｒ大鼠较低，对ＫＣｌ和ＡＣｈAim:To investigate the resting membrane potential(Em) of coronary arterial smooth muscle cells(CASMC) from SHR and the reactivity of Em to KCl, ACh and NE,and compared with that of Wistar rats. Methods: Intracellular micro electrode method was used to measure Em. Results:The resting Em of CASMC was steady. Mean values for Em were -42.40±2.70 mV and were higher 22% than that of Wistar rats; both KCl and ACh caused depolarization of Em in a concentration response manner. NE have no effect on Em. Conclusion: The resting Em of SHR was lower than that of Wistar rats, and the reactivity of Em to KCl and ACh was significantly increased.

关 键 词：高血压 血管平滑肌 膜电位 乙酰胆碱 

分 类 号：R544.1[医药卫生—心血管疾病]