OPG基因敲除小鼠骨量及微结构的改变  

Changes of bone quality and micro-architecture in OPG-knockout mice

作　　者：梁青春[1] 徐康[2] 刘江华[3] 盛志峰[4] 廖二元[4] 

机构地区：[1]中南大学湘雅二医院病理科,湖南长沙410011 [2]中山大学孙逸仙纪念医院普外科,广东广州510000 [3]南华大学附属第一医院内分泌科,湖南衡阳421001 [4]中南大学湘雅二医院内分泌科,湖南长沙410011

出　　处：《中国现代医学杂志》2011年第17期1989-1994,共6页China Journal of Modern Medicine

摘　　要：目的研究小鼠OPG基因敲除对其骨质量及微结构的影响。方法 HE、扫描电镜、四环素荧光及显微CT观察OPG基因敲除小鼠骨微结构的改变;DXA仪测定小鼠股骨标本的总体骨密度和骨矿含量。结果与野生型小鼠相比,OPG基因敲除小鼠松质骨与皮质骨均出现明显的骨丢失,伴显著的骨微结构破坏;总体骨密度和骨矿含量减少。结论 OPG-/-小鼠发生明显骨丢失,骨微结构破坏。[Objective] To investigate the changes of bone quality and micro-architecture in OPG knockout （OPG^-/-） mice. [Methods] Bone micro-architecturc and loss of bone mass were assessed by hematoxylineosin staining, scanning electron microscope and micro-CT. BMD and BMC were detected by DXA. [Resuits] Compared with wild-type mice, OPG4^-/- mice showed a loss of BMD and disturbance of micro-architecture. The BMD and BMC were reduced by DXA determination. [Conclusions] The disturbant bone micro-architecture and lowered BMD were observed in OPG^-/- mice.

关 键 词：骨保护素 基因敲除 显微CT 骨微结构 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]