氟对体外骨器官培养长骨细胞周期和细胞凋亡的影响  

Effects of Fluoride on The Cell Cycle and Apoptosisin Vitro Organ Culture of Long Bone

作　　者：刘开泰[1] 王国荃[1] 汪师贞[1] 吴继尧[1] 肖碧玉[1] 

机构地区：[1]新疆医科大学环境卫生教研室,新疆乌鲁木齐830054

出　　处：《地方病通报》1999年第4期1-3,共3页Endemic Diseases Bulletin

摘　　要：为了探讨氟对体外骨器官培养长骨细胞周期分布和细胞凋亡的影响，我们采用体外骨器官培养的方法．流式细胞术检测长骨细胞周期分布、ＤＮＡ含量和细胞凋亡。结果发现１．染氟２．５～５.０μｇ／ｍＬ，对体外培养长骨细胞ＤＮＡ含量和细胞周期分布影响不大。染氟１０．０μｇ／ｍＬ，Ｓ期细胞数增加，但Ｇ０／Ｇ１期和Ｇ２／Ｍ期细胞无明显改变。染氟２０．０μｇ／ｍＬ，Ｓ期细胞数增加，Ｇ2／Ｍ期细胞数减少，但Ｇ０／Ｇ１期细胞无明显改变。表明氟作为一种刺激因子，干扰了细胞周期正常的信号传导，使Ｓ期的细胞进入Ｇ２／Ｍ期受到抑制，细胞周期停滞在Ｓ期，从而使分裂期细胞减少，导致细胞增殖受到影响。２·染氟浓度在２·５～１０·００μｇ／ｍＬ范围，未见氟对体外培养长骨细胞凋亡有明显影响。氟２０．０μｇ／ｍＬ可诱导体外培养长骨细胞凋亡。结果表明·氟对骨骼的损伤与氟诱导细胞凋亡和骨细胞周期分布异常改变有密切关系。To investigate the effects of fluoride on the cell cycle and apoptosis of longbone, Cell cycle, DNA content and apoptosis were observed with flow cytometry (FLM) byusing the technique of organ culture of bone. Results Shown: 1. The concentrations offluoride at 2. 5~5' Oμg/mL did not influence the DNA contents and cell cycle distributions inin vitro organ culture of long bone. Fluoride at 10. oμg /mL increased the number of cells inS phase, but not changed that in G,/ G, and G,/M phase' Fluoride at 20. 0μg /mL not onlyincreased the number of cells in S phase, but also decreased that in G,/M phase' Fluoride,as a stimulative factor, interrupted the normal signal tranducer and inhibited cell from Sphase to G,/M phase and made cell cycle stop in S phase. Cell proliferation was influenced.2' The concentrations of fluoride at 2' 5~10. Oμg /mL did not induce apoptosis to increase inin vitro, but the number of apoptotlc cells showed a significant increase at 20. 0μg /mL offluoride. It is possible that the damage of fluoride on skeleton may be related with thepromoting apoptosis and disconcerting cell cycle distributions.

关 键 词：氟 骨器官培养 流式细胞术 细胞周期 细胞凋亡 

分 类 号：R995[医药卫生—毒理学]