肺癌组织中NF-κB-p65 mRNA与CD15s蛋白的表达及与细胞凋亡的关系  

Expression of Nf-κB-p65 mRNA and CD15s and Its Correlation with Tumor Cell Metastasis in Lung Cancer Tissues

作　　者：兰四友[1] 蒋幼凡[2] 

机构地区：[1]泸州医学院附属医院呼吸内科,四川泸州646000 [2]重庆医科大学附属第二医院呼吸内科

出　　处：《环境与健康杂志》2011年第6期512-514,共3页Journal of Environment and Health

摘　　要：目的探讨核转录因子κB-p65(NF-κB-p65)、CD15s蛋白在肺癌组织中的表达及其与细胞凋亡的关系,为进一步探索肺癌发生和发展机制提供依据。方法收集手术切除的70例肺癌组织及15例癌旁正常组织标本,分别用RT-PCR方法、TUNEL法、免疫组织化学染色法检测肺癌组织及癌旁组织中NF-κB-p65mRNA的表达、凋亡指数及组织中CD15s蛋白表达情况。结果 NF-κB-p65 mRNA在肺癌组织中的表达高于癌旁正常肺组织(P<0.05),其中在腺癌组织表达较强,小细胞癌组织表达次之,鳞癌组织表达较弱,前两者表达强于后者(P<0.05)。肺癌组织中的细胞凋亡指数[(5.40±0.72)%]低于癌旁正常肺组织[(9.39±0.42)%],差别有统计学意义(P<0.05)。肺癌组织中NF-κB-p65mRNA表达水平和细胞凋亡指数呈负相关(r=-0.532,P<0.001)。肺癌组织中CD15s蛋白的表达阳性率为64.3%,15例癌旁正常肺组织CD15s蛋白的表达均为阴性。不同病理类型、不同淋巴结转移情况、不同分化程度、不同TNM分期的肺癌组织NF-κB-p65 mRNA和CD15s蛋白的表达差异有统计学意义(P<0.05)。结论 NF-κB-p65和CD15s可能在肺癌浸润、转移过程起促进作用。NF-κB-p65的表达与凋亡指数呈负相关,前者可能促进肺癌细胞的分化、增殖,在肺癌中发挥抗凋亡作用。Objective To study the expression of nuclear factor（NF）-κB-p65 and CD15s in lung cancer tissues,and to explore the relationship between the expression and tumor cell metastasis and infiltration and tumor apoptosis,and to explore the possible molecular mechanism of lung cancer cell metastasis and infiltration.Methods Seventy samples of lung cancer tissues and 15 normal lung tissues were collected through surgical operation.RT-PCR method was used to detect the expression of NF-κB-p65mRNA in the lung cancer tissues and lung tissues around tumor.Cell apoptosis index in tissues was detected by the TUNEL method.The expression of CD15S was detected in these tissues with Immunohistochemistry.Results The expression of NF-κB-p65mRNA in the lung cancer tissues was significantly higher than that in the lung tissues around tumor.The expression of NF-κB-p65 mRNA in adencarcinoma tissues was higher than that in small cell lung cancer and squamous carcinoma tissues,the expression in three groups had significant difference（P0.05）.Cell apoptotic index in lung cancer tissues was significantly lower than that in lung tissues around tumor（P0.05）.The expression of NF-κB-p65mRNA was negative correlated with apoptotic index in the lung cancer tissues.The positive rate of CD15s was 64.3%,but the expression of CD15s in 15 cases of the lung tissues around tumor was negative.The difference of expression in the two groups was significant（P0.05）.The expression of the NF-κB-p65 mRNA and CD15s protein was associated with the tumor cell differentiation,the lymph node metastasis,TNM staging and pathological typing.Conclusion NF-κB-p65 and CD15s may facilitate the lung cancer cell metastasis and infiltration;NF-κB-p65 may enhance the lung cancer cell differentiation and proliferation,and play an anti-apoptotic role in lung cancer.

关 键 词：肺癌 核转录因子κB-p65 CD15S 转移 

分 类 号：R994.6[医药卫生—毒理学]