透皮促进剂对左旋延胡索乙素体外经皮渗透的影响  

Influence of penetration enhancers on in vitro transdermal permeation of L-tethrahydropalmatine

作　　者：钱励[1] 马臻[1] 张望刚[1] 王俏[1] 

机构地区：[1]浙江省医学科学院药物研究所,浙江杭州310013

出　　处：《中国中药杂志》2011年第13期1729-1732,共4页China Journal of Chinese Materia Medica

基　　金：浙江省科技计划项目(2007F30013)

摘　　要：目的:考察不同透皮促进剂对左旋延胡索乙素体外经皮渗透的影响。方法:采用Valia-Chien双室渗透扩散池,以大鼠离体皮肤为渗透屏障,用高效液相色谱法对左旋延胡索乙素进行含量测定,考察化学类、天然类渗透促进剂单用和合用对左旋延胡索乙素的促透效果。结果:化学类促进剂单用时,8%氮酮促透效果最佳,平均渗透速率达21.153μg.cm-2.h-1。天然类促进剂单用时,2%薄荷醇和5%桉叶油促透效果较佳,但平均渗透速率约为8%氮酮的一半。促进剂合用时,薄荷油或薄荷醇与8%氮酮合用的促透效果较好,均比单用8%氮酮有显著性增加(P<0.05)。结论:薄荷油或薄荷醇与8%氮酮合用对左旋延胡索乙素饱和溶液体外渗透具有较好的促透效果,天然类和化学类渗透促进剂合用具有良好的加和作用。Objective： To study the effect of different penetration enhancers on the in vitro transdermal permeation of l-tethrahydropalmatine（L-THP） through rat skin.Method： Both natural and chemical synthesis penetration enhancers were applied singly or jointly to investigate the skin permeation rates of l-THP.The skin permeation profiles were evaluated by Valian-Chien permeation cells with isolated rat skin.HPLC-UV method was established to determine the concentration of l-THP in samples.Result： As chemical synthesis penetration enhancer was used alone,8% azone was observed to be the optimal penetration enhancer with the maximal penetration rate of 21.153 μg·cm-2·h-1.Although 2% menthol crystal or 5% eucalyptus oil was effective as a natural penetration enhancer when used alone,the average penetration rate reached only half of that of 8% azone.The penetration potency of either menthol oil or menthol crystal combined with 8% azone was more effective than that of azone alone（P0.05）.Conclusion： Either menthol oil or menthol crystal combined with 8% azone is effective on transdermal penetration of l-THP in vitro.There is significant synergistic effect when natural penetration enhancers combined with chemical synthesis penetration enhancers.

关 键 词：左旋延胡索乙素 经皮渗透 促进剂 氮酮 薄荷油 薄荷醇 

分 类 号：R285.5[医药卫生—中药学]