条斑紫菜低覆盖度基因组草图分析  

The analysis of the low coverage Porphyra yezoensis draft genome

作　　者：牛建峰[1] 高胜寒[2] 骆迎峰[2] 袁野[2] 王广策[1] 胡松年[2] 

机构地区：[1]中国科学院海洋研究所,山东青岛266071 [2]中国科学院北京基因组研究所,北京100029

出　　处：《海洋科学》2011年第6期76-81,共6页Marine Sciences

基　　金：国家863计划项目(2007AA09Z406);国家自然科学基金项目(30830015);国家973计划前期专项(2011CB411908)

摘　　要：对条斑紫菜(Porphyra yezoensis)进行Solexa高通量测序,获得低覆盖度全基因组草图。该基因组草图大小约220 Mbp,GC质量分数53.08%;包含26 629个预测基因,其中16 409个基因具有内含子,平均每个基因含2.22个内含子;基因结构分析表明具有内含子的基因平均长度为2 214 bp,内含子平均大小319 bp;代谢通路分析表明嘌呤代谢是含有蛋白数量最多的代谢途径。本研究所得条斑紫菜低覆盖度全基因组草图快速获取了基因组大小和蛋白编码基因结构等基本信息,证明了使用Solexa高通量测序技术对条斑紫菜进行全基因组测序的可行性。We constructed the low-coverage Porphyra yezoensis draft genome using the Solexa high-throughput sequencing system. The predicted genome size was approximately 220 Mbp, with the GC content of 53.09%. The draft genome contained 26 629 predicted protein-coding genes, 16 409 of which had introns, averagely 2.22 introns per gene. Gene structure analysis of intron-containing genes showed that the average gene size and intron size was 2214 bp and 319 bp, respectively. Metabolic pathway analysis indicated that the purine metabolism was the largest pathway. The P.yezoensis draft genome constructed in this study manifested important genomic properties such as genome size and the gene structure of protein-coding gene, proving the feasibility of Solexa high-throughput system in sequencing the whole genome of P.yezoensis.

关 键 词：条斑紫菜(Porphyra yezoensis) Solexa高通量测序 代谢通路 

分 类 号：Q949.21[生物学—植物学] Q942.4