马蔺种质材料过氧化物酶同工酶酶谱特征分析  

Isozyme band analysis of Chinese Iris germplasms

作　　者：李强栋[1,2] 孟林[2] 毛培春[2] 张德罡[1] 韩润英[3] 

机构地区：[1]甘肃农业大学草业学院,甘肃兰州730070 [2]北京市农林科学院北京草业与环境研究发展中心,北京100097 [3]内蒙古通辽市畜牧兽医科学研究所,内蒙古通辽028000

出　　处：《草业科学》2011年第7期1331-1338,共8页Pratacultural Science

基　　金：北京市自然科学基金项目(6102013);北京市科技计划项目(Z09090501040902);农业部牧草种质资源保护项目

摘　　要：采用非连续垂直聚丙烯酰胺凝胶电泳技术,对我国野生分布的23份马蔺(Iris lactea var.chinensis)种质材料进行过氧化物酶(POD)同工酶谱特征分析。结果显示,在相对迁移率(Rf)为0.041～0.515的位点处,共有30个位点检测出谱带,谱带数为5～9条,其中BJCY-ML004和BJCY-ML024谱带数最多(9条),而BJCY-ML001、BJCY-ML018、BJCY-ML021、BJCY-ML029谱带数最少(5条),表现出谱特征差异较大,说明POD同工酶具多态性,可作为遗传标记用于种质材料遗传多样性检测。POD同工酶相似性系数值范围为0.516～1.000。BJCY-ML004和BJCY-ML020之间相似性系数最小(0.516),BJCY-ML028和BJCY-ML031之间相似性系数最大(1.000),其他材料间的相似性系数为0.548～0.968,说明来自不同居群马蔺种质材料有极近的亲缘关系。聚类分析进一步表明,当相似性水平为0.71时,可将参试的23份种质材料划分为4个一级类群,也充分反映了不同居群马蔺种质材料与其地理分布和生境间存在一定的相关关系。POD isozyme banding patterns of were identified for 23 wild Chinese Iris germplasms by means of vertical polyacrylamide gel electrophoresis. This study showed that the relative mobility （Rf） located at 0. 041 to 0. 515 loci, and the band numbers ranged from 5 to 9, in which BJCY-ML004 and BJCY-ML020 band numbers were the most （9）, BJCY-ML001 ,BJCY-ML018,BJCY-ML021 ,BJCY-ML029 band numbers were the least （5）. The genetic similarity of POD ranged from 0. 516 to 1. 000, and the genetic similarity between BJCY-ML004 and BJCY-ML020 was the lowest （0. 516） and those between BJCY-ML028 and BJ- CY-ML031 was the highest （1. 000）. The genetic similarity of other germplasms was from 0. 548 to 0. 968, indicating that there were dose genetic similarity of all germplasms. Cluster analysis showed that the 23 wild Chinese Iris were classified into 4 first-class groups when the genetic similarity was 0.71, sug- gesting that the Iris lactea vat. chinensis communities were related to their geographical distribution and ecological habitats.

关 键 词：马蔺 POD 聚类分析 遗传多样性 亲缘关系 

分 类 号：S682.19[农业科学—观赏园艺]