丹参提取液对球形红细菌生长的影响  

Effects of Salvia miltiorrhiza extract on growth of Rhodobacter sphaeroides

作　　者：张辉[1] 李建文[1] 宋艳红 杨官娥[1] 

机构地区：[1]山西医科大学药学院,山西太原030001 [2]山西省兽药监察所,山西太原030027

出　　处：《中草药》2011年第7期1413-1416,共4页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(30672621);山西省自然科学基金资助项目(2010011048-2)

摘　　要：目的考察丹参提取液对球形红细菌(光合细菌)生长的影响。方法通过添加不同质量浓度的常规培养基和丹参提取液,培养球形红细菌,考察球形红细菌的活菌数、生长曲线、脱氢酶活性及光合色素吸收光谱,研究丹参提取液对球形红细菌生长的影响。结果培养基中添加丹参提取液后可使球形红细菌活菌数增加到之前的1.6倍,并缩短球形红细菌生长的延迟期,提前进入指数期及稳定期,可使球形红细菌脱氢酶活性提高到之前的6.6倍。低质量浓度丹参提取液基本不影响球形红细菌中光合色素的形成,高质量浓度时吸收均变低,且影响类胡萝卜素的形成,其红外光谱855 nm处红移至863 nm。结论培养基中添加丹参提取液可影响球形红细菌生长,缩短球形红细菌生长周期,提高球形红细菌的活力,高质量浓度时影响光合色素的形成。Objective To study the effects of Salvia miltiorrhiza extract on the growth of Rhodobacter sphaeroides（photosynthetic bacteria,PSB）.Methods The effects of S.miltiorrhiza extract on the growth of R.sphaeroides were researched by observing the effects of S.miltiorrhiza and general culture medium at concentration on the number,growth curve,dehydrogenase activity,and absorption spectra of photosynthetic pigment of alive R.sphaeroides.Results After adding S.miltiorrhiza extract into culture medium for 3 d,the number of alive R.sphaeroides was increased in 1.6 times,the delay period was shortened and the expotential and stationary periods were advanced which could shorten the growth period of R.sphaeroides,the dehydrogenase activity of R.sphaeroides was improved in 6.6 times as before.When S.miltiorrhiza concentration was higher,the absorption of photosynthetic pigment of alive R.sphaeroides was getting lower,which was almost insusceptible while S.miltiorrhiza concentration was lower.When S.miltiorrhiza concentration was high,the formation of carotenoid which was in short wavelength region was influenced and the peak at 855 nm was approximately blue-shifted to 863 nm.Conclusion When adding S.miltiorrhiza extract into culture medium,the growth and the activity of R.sphaeroides could be improved,the growth cycle be shortened and the formation of photosynthetic pigment be effected.

关 键 词：丹参 光合细菌 球形红细菌 脱氢酶活性 光合色素 

分 类 号：R282.21[医药卫生—中药学]