麻疯树根边缘细胞生物学特性及镉对其活性的影响  

Biological characteristics of Jatropha curcas root border cells and toxic effect of cadmium on the cell viability

作　　者：梁剑[1,2] 刘小文[3] 唐琳[1] 徐莺[1] 王胜华[1] 陈放[1] 

机构地区：[1]四川大学生命科学学院生物资源与生态环境教育部重点实验室,成都610064 [2]西昌学院农业科学学院,四川西昌615013 [3]云南农业大学植物保护学院,昆明650201

出　　处：《生态学杂志》2011年第7期1423-1428,共6页Chinese Journal of Ecology

基　　金：国家"十一五"科技支撑计划课题项目(2006BAD07A04);四川省教育厅科研基金项目(2009ZC006)资助

摘　　要：以麻疯树为试材,采用悬空气法,探讨麻疯树离体根边缘细胞的生物学特性及重金属镉(Cd2+)对根边缘细胞活性的影响。结果表明,麻疯树根边缘细胞大多数呈椭圆型,少数成弯曲的长条型;根长为15 mm时,边缘细胞的生物学活性(存活率)最大;根冠果胶甲基脂酶(PME)酶活性随着根的伸长有所降低。镉对麻疯树根边缘细胞产生明显毒害,随着镉浓度和处理时间的增加,边缘细胞的活性都呈现下降的趋势。Jatropha curcas root border cells were detached from root tip and cultured by aeroponics to study their biological characteristics and the toxic effect of cadmium on their viability.Most of the cells were elliptical,and a few of them presented bend claval shape.The cells had the highest viability when the root length was 15 mm,and their pectin methylesterase（PME） activity somewhat decreased with root elongation.Cadmium had obvious toxic effect on the cells.With the increasing concentration of cadmium and the increasing treating time,the viability of the border cells all showed a decreasing trend.

关 键 词：镉毒 麻疯树 胁迫反应 离体根边缘细胞 

分 类 号：S792.99[农业科学—林木遗传育种]