吴茱萸提取物对大鼠右室肥大及钙调神经磷酸酶的抑制作用研究  

Inhibitory Effect of the Extracts of Euodiae Fructus on Right Ventricular Hypertrophy and Calcineurin of Rats

作　　者：高永双 孙安盛[2] 何娜[2] 吴芹[2] 杨丹莉[2] 

机构地区：[1]广东中山市第三人民医院药剂科,中山市528451 [2]遵义医学院药理学教研室/贵州省基础药理重点实验室,遵义市563003

出　　处：《中国药房》2011年第27期2508-2510,共3页China Pharmacy

基　　金：2009年度贵州省中医药管理局中医药;民族医药科学技术研究课题(D-360)

摘　　要：目的:研究吴茱萸提取物(EV)对野百合碱(MCT)所致大鼠右室肥大及钙调神经磷酸酶的抑制作用。方法:将60只雄性Wistar大鼠均分为正常对照、右室肥大模型、硝苯地平(20mg·kg-1)和EV低、中、高剂量(50、100、200mg·kg-1)组。连续ig给药18d,测定大鼠右室肥大指数、右室相对质量和肺重/体重,实时定量PCR检测心肌组织钙调神经磷酸酶(CaN)mRNA的表达,免疫组化染色测定CaN催化亚基(CnA)的蛋白表达。结果:与模型组比较,EV50、100、200mg·kg-1使右室肥大指数、右室相对质量和肺重/体重显著降低(P<0.01或P<0.05),CaNmRNA和CnA的蛋白表达显著降低(P<0.01)。结论:EV能明显改善MCT引起的大鼠右心室肥大,其机制可能与其抑制CaN信号转导通路有关。OBJECTIVE：To observe the inhibitory effect of the extracts of Euodiae Fructus（EV） and monocrotaline（MCT） on right ventricular hypertrophy（RVH） and calcineurin of rats.METHODS：60 male Wistar rats were randomly divided into 6 groups：normal control group,right ventricular hypertrophy model group,nifedipine（20 mg·kg-1）group,Euodiae Fructus.rutaecarpa low-dose,medium-dose and high dose groups（50,100,200 mg·kg-1）.The rats were given drugs intragastrically for 18 days.RVH index,RV weigh/body weigh（RVW/BW）,lung weight/ body weigh（LW/ BW） of rats were measured.The expressions of calcineurin（CaN） mRNA and CnA protein were separately determined by real-time RT-PCR and immunohistochemistry.RESULTS：Compared with model group,Euodiae Fructus（50,100,200 mg·kg-1） could significantly decrease RVH index,RVW/BW and LW/ BW of rats（P0.01 or P0.05）,at the same time the expressions of CaN mRNA and CnA protein were significantly suppressed by Euodiae Fructus（P0.01）.CONCLUSION：Euodiae Fructus can significantly attenuate the right ventricular hypertrophy induced by MCT in rats,its mechanism may be associated with inhibition effect of it on CaN signal transduction pathway.

关 键 词：大鼠 心室肥大 吴茱萸提取物 钙调神经磷酸酶 

分 类 号：R285[医药卫生—中药学] R97[医药卫生—中医学]