幽门螺杆菌唾液测试板与^(14)C-尿素呼气试验检测幽门螺杆菌感染的比较  

Comparison of the Hp saliva test plate and ^(14)C urease breath test for detecting Hp infection

作　　者：楼晓军[1,2] 王彩花[1] 朱永良[1] 温晓伟[3] 马德发[2] 

机构地区：[1]浙江大学医学院附属第二医院消化科,杭州市310009 [2]浙江省嘉兴市第一医院消化科,314000 [3]浙江省嘉兴市第一医院病理科,314000

出　　处：《实用医学杂志》2011年第13期2356-2358,共3页The Journal of Practical Medicine

摘　　要：目的:比较幽门螺杆菌唾液测试板(HPS)与14C-尿素呼气试验(14C-UBT)检测幽门螺杆菌(Hp)感染的临床价值。方法:用HPS检测唾液中的尿素酶,同时行14C-UBT及胃镜检查行快速尿素酶试验(rapidurease test,RUT)、胃黏膜组织学检查(改良Giemsa染色)及细菌培养联合检测来判断胃黏膜Hp感染情况,并比较HPS与14C-UBT诊断Hp感染的阳性率、敏感性、特异性、准确性、阳性预测值及阴性预测值。结果:160例患者行Hp联合检测检出阳性患者102例,阳性率为63.75%,其中HPS检出76例,阳性率47.50%,14C-UBT检出94例,阳性率58.75%。HPS、14C-UBT诊断Hp感染的敏感性分别为72.55%、80.39%;特异性分别为96.55%、79.31%;准确性分别为81.25%、80.00%;阳性预测值分别为97.37%、87.23%;阴性预测值分别为66.67%、69.70%。对以上结果进行χ2检验,差异均无统计学意义。结论:HPS检测Hp感染的阳性率及准确性、敏感性、特异性与14C-UBT相近,且此法快速、简便,价格低廉,不需要任何其他仪器,是值得临床进一步验证的一种新的非侵入性Hp感染诊断方法。Objective To investigate the clinical value of the Hp saliva test plate （HPS） and 14C urease breath test （14C-UBT） for Hp infection. Methods Urease was detected in saliva of patients by means of HPS, and Hp infection were detected in the biopsy specimen of gastric mucosa with 14C-UBT and the combined detection of RUT, modified Giemsa staining and bacterial culture at the same time. Then comparisons were made between HPS and 14C-UBT groups by the positive rate, sensitivity, specificity, and the positive （or negative） predictive value. Results 102 from 160 patients were diagnosed as positive for Hp infection by a combined method, and the positive rate was 63.75%. Among them, the positive rates of HPS and 14C-UBT were 47.50%, 58.75%, respectively; while the sensitivity is 72.55 % for diagnosing Hp infection by HPS, 80.39 % by 14C- UBT; The specificity, the correct rate, the positive and the negative values were 96.55%, 81.25%, 97.37% and 66.67% by HPS; 80.00%, 87.23%, 79.31%, 69.70% by 14C-UBT, but the difference between them was not statistically significant. Conclusions The positive rate, correct rate, sensitivity and specificity for detecting urease in saliva by HPS are similar to them of 14C-UBT. The method is rapid, simple, cheap, and worthy of further clinical validation as a new non-invasive diagnostic method for Hp.

关 键 词：螺杆菌 幽门 唾液 呼气试验 检测方法 

分 类 号：R817.4[医药卫生—影像医学与核医学]