滋补脾阴方药对脾阴虚痴呆大鼠海马蛋白质组影响的研究  

Empirical Study on Proteome of Hippocampus from Alzheimer's Disease Rat Model with Spleen Yin Deficiency Syndrome Treated by Zi-Bu-Pi-Yin Recipe

作　　者：战丽彬[1,2] 刘莉[3] 路小光[3] 宫晓洋[4] 梁丽娜[1,2] 施翔[1,2] 

机构地区：[1]大连医科大学附属二院,大连116023 [2]大连医科大学中西医结合研究院,大连116044 [3]大连大学附属中山医院,大连116001 [4]大连医科大学附属一院,大连116011

出　　处：《世界科学技术-中医药现代化》2011年第3期480-487,共8页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金课题(30472255):滋补脾阴方药对兴奋性传递中树突棘调控的分子机制研究,负责人:战丽彬

摘　　要：目的:从蛋白质组水平探索脾阴虚痴呆的本质以及滋补脾阴的方药(ZBPYR)作用靶点。方法:采用饮食不节、劳倦过度、耗伤阴液的复合因素综合考虑的方法建立脾阴虚的大鼠模型。并于双侧海马定位注射聚合态β-淀粉样蛋白1-40,应用ZBPYR干预。通过双向凝胶电泳比较各组大鼠海马蛋白质组表达图谱的不同;由MALDI-TOF-MS鉴定表达差异的蛋白。结果:与空白对照组相比,脾阴虚痴呆组有9个蛋白表达差异点;脾阴虚痴呆+ZBPYR组有2个差异点。经质谱鉴定显示脾阴虚痴呆组大鼠海马蛋白中AnnexinⅢ表达上调,Tubulin beta chain 15、Dihydropyrimidinase-like 2和Guanine nucleotide-binding protein beta-1 subunit表达下降,ZBPYR干预后这些蛋白点的表达与空白对照组比较无明显差异。结论:脾阴虚痴呆大鼠海马的病变是一个多种蛋白质参与的复杂过程,AnnexinⅢ、Tubulin beta chain 15等分子参与了脾阴虚痴呆大鼠的海马病变;ZBPYR可能通过对海马的差异表达蛋白的调控而达到治疗目的。This study aimed to find out the disease nature of Alzheimer＇s disease （AD） with spleen yin deficiency syndrome and search for effective therapeutic targets of Zi-bu-pi-yin Recipe （ZBPYR） at proteome level. The rat model of spleen yin deficiency was established through means of improper diet, overstrain and consumption of body fluid. Then, the polymerized β-Amyloid 1-40 was injected into hippocampus on both sides of model rats. There were obvious differences in the expression of two protein spots between the ZBPYR-treated group and normal control group. Changes of some proteins expression levels in the hippocampus of rats may contribute to AD with spleen yin deficiency syndrome. The good effect of ZBPYR in the treatment of AD with spleen yin deficiency syndrome may through the regulation on protein expression in hippoeampus tissue of rats.

关 键 词：脾阴虚痴呆 证病结合 滋补脾阴方药 蛋白质组学 海马 

分 类 号：R651.2[医药卫生—外科学]