人参皂苷抗金葡菌肺感染的作用  

Anti-Infection of Ginsenosides on Staphylococcus aureus both In Vitro and In Vivo

作　　者：柴玉爽[1] 孟甄[1] 兰嘉琦[1] 吴昊[1] 雷帆[1] 万红娇[2] 袁梽漪[1] 邢东明[1] 杜力军[1] 

机构地区：[1]清华大学生命科学学院医学院药物药理研究室,北京100084 [2]江西中医学院,南昌330004

出　　处：《世界科学技术-中医药现代化》2011年第3期509-517,共9页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金资助项目(30973896):从黄芩苷对TLRs-NOD2模式识别及相关信号转导通路的调控探讨肺;脑相关炎性病变的共有机制;负责人:邢东明;科学技术部国家"重大新药创制"科技重大专项(2009ZX09502):与药效相关的中药质量评价关键技术研究;子课题负责人:杜力军;科学技术部"重大新药创制"科枝重大专项(2009ZX09308-003-33):中药生产技术及过程控制技术标准平台;子课题负责人:雷帆

摘　　要：目的:观察研究人参皂苷对金葡菌感染的作用及其特点。方法:体外肺AⅡ型上皮细胞及小鼠肺感染为模型,观察金葡菌感染后细胞侵染相关因子、炎性相关因子以及细菌数和死亡率,以及人参皂苷及人参皂苷Rg1的作用。结果:金葡菌感染后,人参皂苷能够明显降低胞内菌含量,降低粘附因子及其整合素的表达,抑制相关炎性因子,人参皂苷Rg1是其主要活性成分。结论:在抗金葡菌急性感染过程中,人参皂苷发挥了对感染细胞和组织的保护作用,而这些作用不完全依赖于机体免疫系统的功能。This study aimed to evaluated the anti-infective effect of ginsenosids to Staphylococcus aureus （S. aureus） both in vitro and in vivo. Based on the model of rat pulmonary epithelial cells in vitro and rat infection in vivo, the alteration of cytokines, proinflammatory factors, adhesion factors, and bacterial count were studied. The results showed that ginseno- sides can significantly reduce the bacteria count both within the cell and in the lungs after S. aureus infection. The expression of cytokines, proinflammatory factors, imegrin and other adhesion factors were also down-regulated. Ginsenoside Rgl was the major active component of the saponins. It is concluded that the immunomodulation-independent action of ginseng is through reducing adhesion of S. aureus to epithelial cells, down-regulating expression of integrin, and alleviating inflammatory responses, by which initiate the protection of the host from the damage of S. aureus.

关 键 词：人参皂苷金葡菌 感染 

分 类 号：R284.1[医药卫生—中药学]