不同程度间歇低氧对大鼠血清炎性细胞因子白介素8、白介素10的影响  

Effects of different degree of intermittent hypoxia on serum levels of inflammatory cytokine interleukin-8 and interleukin-10 in rats

作　　者：李硕[1] 陈宝元[1] 用伟[1] 万南生[1] 张祯[1] 郭润[1] 

机构地区：[1]天津医科大学总医院呼吸科,300052

出　　处：《国际呼吸杂志》2011年第13期999-1003,共5页International Journal of Respiration

基　　金：基金项目：国家自然科学基金资助项目（30770934,30900656）

摘　　要：目的探讨不同程度间歇低氧对大鼠血清炎性细胞因子白介素8（IL-8）和抗炎细胞因子IL-10水平的影响。方法160只雄性Wistar大鼠随机分为5组：5％，7．5％，10％间歇低氧组，10％持续低氧对照组和常氧对照组各32只，分别于低氧暴露第2周、第4周、第6周和第8周每组随机抽取8只大鼠，应用酶联免疫吸附试验法检测血清IL-8和IL-10浓度。结果各间歇低氧组和持续低氧组血清IL-8水平均随低氧时间呈明显升高（F=6．42，P〈0．01），IL-10水半明显降低（F=5．787，P〈0．01）。在低氧6周时所有间歇低氧组出现明显的峰值，5％，7．5％，10％间歇低氧组IL-8水平明显高于10％持续低氧对照组和常氧对照组（P值均〈0．01），而IL-10水平明显低于10％持续低氧对照和常氧对照组（P〈0．01）。其中5％间歇低氧组血清IL-8水平明显高于10％间歇低氧组（F=34．68，P=0．046）。结论间歇性低氧和持续性低氧均可造成促炎性细胞因子IL-8的升高和抗炎性细胞因子IL-10的降低。间歇低氧可能比持续低氧引起更严重的炎性损伤反应，且炎性因子的释放存在明显的低氧程度依赖性和低氧时间依赖性。Objective To investigate the effects of different degree of intermittent hypoxia on serum levels of pre-inflammatory cytokines interleukin-8 （IL-8） and anti inflammatory cytokines IL-10 in rats. Methods One hundred and sixty male Wistar rats were divided randomly into the five groups： 5%, 7.5%,10% of intermittent hypoxia group,10% sustained hypoxia control group and the normal oxygen control group. At the second, fourth, sixth, and eighth weeks, 8 rats under different environmental stimulation hypoxia in each group were sacrificed to collect serum. Enzyme Linked Immuno Sorbent Assay （ELISA） assay were used to detect the concentration of serum IL-8 and IL -10. Results Serum level of IL- 8 was found to be significantly increased in intermittent hypoxia and sustained hypoxia groups （ F= 6.42, P 〈0.01） with progression of time, while level of IL-10 was significantly decreased （ F =5. 787, P〈0.01）. We identified the peak for serum levels of IL-8 and IL -10 in the sixth week in all three intermittent hypoxia groups. Serum levels of IL- 8 were higher in 5%, 7.5%, 10% of intermittent hypoxia group than in 10% sustained hypoxia control group and the normal oxygen control group groups （ P 〈0.01）, whereas levels of IL-10 were significantly lower than 10% sustained hypoxia control group and the normal oxygen control group groups （ P 〈0.01）. Level of IL- 8 in 5% intermittent hypoxia group was significantly higher than those in 10% intermittent hypoxia group group （ F = 34.68, P = 0. 046）. Conclusions Intermittent hypoxia and sustained hypoxia can result in elevation of pro inflammatory cytokines IL-8 and descent of anti-inflammatory cytokine IL- 10. Inflammatory response induced by intermittent hypoxia had more serious implications than that by 10% sustained hypoxia control group. Furthermore the release of inflammatory factors is associated with the degree and time of intermittent hypoxia.

关 键 词：间歇低氧 阻塞性睡眠呼吸暂停综合征 白介素8 白介素10 

分 类 号：R766[医药卫生—耳鼻咽喉科]