广东地区空调冷却塔水嗜肺军团菌分离株脂肪酸分型研究  

Cellular fatty acid analysis of Legionella pneumophila in air conditioning cooling water in Guangdong

作　　者：王峥[1,2] 陈经雕[2] 宋曼丹[2] 邓小玲[2] 柯碧霞[2] 刘美真[2] 杨杏芬[2] 谷文喜[1,2] 

机构地区：[1]中山大学公共卫生学院,广东广州510080 [2]广东省疾病预防控制中心微生物检验所,广东广州510300

出　　处：《中国病原生物学杂志》2011年第6期424-427,407,共5页Journal of Pathogen Biology

基　　金：广东省科技计划项目(No.2008B030301358)

摘　　要：目的探讨脂肪酸分析方法在种水平上对嗜肺军团菌的判断能力;将脂肪酸分型方法与传统的血清分型方法进行比较,了解不同血清型嗜肺军团菌株脂肪酸成分的异同。方法选取近3年从广东地区空调冷却塔水中分离到的112株嗜肺军团菌分离株,提取脂肪酸,利用MIDI公司Sherlock系统进行菌体脂肪酸成分分析,使用SPSS 13.0软件对获得的数据资料进行统计分析及聚类分析。结果脂肪酸分析法判定嗜肺军团菌的符合率为84.8%,所有菌株共有的脂肪酸成分有9种,主要脂肪酸成分有4种,分别为16∶0 iso、15∶0 anteiso、16∶1 w7c和14∶0 iso脂肪酸。血清1型菌株与血清6、7型菌株相比,有3种主要脂肪酸含量差异有统计学意义(P<0.05)。结论可以依据菌体脂肪酸分析方法对嗜肺军团菌进行快速鉴定;嗜肺军团菌菌株的脂肪酸成分稳定,但各血清型间脂肪酸含量存在一定差异;MIDI数据库中军团菌脂肪酸数据仍有待完善。本研究同时提供了广东地区空调冷却塔水嗜肺军团菌株脂肪酸组成及分型数据。Objective To evaluate the value of cellular fatty acid （CFA） analysis of Legionella pneumophila （Lp） strains to identify species, to compare CFA analysis and serotyping, and to ascertain the differences in CFAs among different Lp serotypes. Methods One hundred and twelve Lp strains belonging to 7 serotypes were isolated from cooling tower water in Guangdong over the last 3 years. The CFA components were extracted and analyzed with the MIDI Sherlock system and SPSS 13.0 statistical software was used for data analysis and cluster analysis. Results Concordance among species was 84. 8%. Nine fatty acids were components common to all of the tested strains. The main fatty acid components were found to be 16 ： 0 iso acid, 15 ： 0 anteiso acid, 16 ： 1 w7c acid, and 14 ： 0 iso acid. Differences between the serotype 1 strain and serotype 6 and 7 strains in terms of the three main fatty acid components were statistically significant. Conclusion CFA analysis was found to help with rapid identification of Lp strains. The fatty acid components of Lp were stable, but the fatty acid components differed among the different serotypes. CFA information for Lp in the MIDI database still needs to be improved.

关 键 词：嗜肺军团菌 气相色谱 脂肪酸分型 聚类分析 

分 类 号：R378.99[医药卫生—病原生物学]