电针对阿尔茨海默病大鼠学习记忆及NOS、CaMKⅡ表达的影响  

The Influence of Electroacupuncture Therapy on the Expression of Hippocampus NOS,CaMKⅡ in Rats' Models of Alzheimer's Disease

作　　者：张晓东[1] 刘洋[1] 王吉锡[1] 姜国华[1] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《针灸临床杂志》2011年第6期70-74,共5页Journal of Clinical Acupuncture and Moxibustion

基　　金：黑龙江省教育厅2008度科学技术研究面上项目;编号:11531339;黑龙江中医药管理局课题;编号:ZHY06-Z20

摘　　要：目的:观察电针对阿尔茨海默病(AD)学习记忆及海马组织内NOS、CaMKⅡ阳性神经元表达的影响,探讨针灸治疗AD的作用机理。方法:选取健康W istar雄性大鼠,随机分为正常组、模型组、生理盐水组、西药组及电针组。采用微量加样器注射每侧侧脑室10%链脲霉素10 ul建立AD模型,模型对照组以等量的生理盐水代替STZ注射。电针组经电针刺激"百会"、"大椎"、"足三里"、"太溪"、"肾俞"穴进行治疗。用Morris水迷宫检测大鼠的行为学改变,用免疫组化法检测海马中NOS、CaMKⅡ阳性神经元表达。结果:与正常组、电针组比较,模型组大鼠学习记忆能力减退,海马区NOS、CaMKⅡ阳性神经元表达明显减少(P<0.01)。经电针治疗后学习记忆能力增强,NOS、CaMKⅡ阳性神经元表达较模型组增加。结论:电针治疗能改善AD大鼠学习记忆能力,增加海马区NOS、CaMKⅡ阳性神经元表达。Objective：To observe the influence of electroacupuncture therapy on the expression of hippocampus NOS,CaMKⅡ in rats＇ models of Alzheimer＇s Disease.Methods：All animals were randomly divided into five groups：normal group,model group,saline group,western medicine group and electroacupuncture group.AD rats were induced by injecting 10% STZ 10ul into the lateral ventricle.Model control group was injected with normal saline instead of STZ.After modeling,electropuncture group after electropuncture stimulation ：＂ Baihui＂,＂Dazhui＂,＂Shenshu＂,＂Zusanli＂,＂Taixi＂ points for treatment.With the mooris water maze test of learning and memory in rats,one randomly selected 50 ratrs to observe by immunohistoochemistry in hippocampus of rats CaMKⅡ,NO expression.Results：Rats＇ learning and memory abilities of model group were significantly lower than that of the normal group and electroacupuncture group（P0.05）.The expressions of NOS,CaMKⅡ in rat hippocampus of model group were significantly lower than those of normal group and elecctroacupuncture group（P0.01）.Conclusion：Electroacupuncture can increase the expression of hippocampus NOS,CaMKⅡ and thereby can prevent a decline in learning and memory abilities in AD rats.

关 键 词：阿尔茨海默病 电针 学习记忆 NOS CaMKⅡ 

分 类 号：R245.97[医药卫生—针灸推拿学]