家蚕中肠组织感染质型多角体病毒的应答基因分析  

Analysis of Responsive Genes in the Midgut of Silkworm Infected with Bombyx mori Cytoplasmic Polyhedrosis Virus

作　　者：吴萍[1,2] 刘挺[1,2] 覃光星[1,2] 郭锡杰[1,2] 

机构地区：[1]江苏科技大学 [2]中国农业科学院蚕业研究所,江苏镇江212018

出　　处：《中国农业科学》2011年第13期2814-2822,共9页Scientia Agricultura Sinica

基　　金：国家自然科学基金项目(30972143);江苏省自然科学基金(BK2010353)

摘　　要：【目的】筛选家蚕(Bombyx mori)中肠感染质型多角体病毒的应答基因,为阐明家蚕感染质型多角体病的分子机制奠定基础。【方法】应用含有约23 000个家蚕基因组寡核苷酸芯片比较分析感染家蚕质型多角体病毒24、48及72 h的中肠试验组与中肠对照组的差异表达基因;结合生物信息学工具对差异表达基因的功能注释、分类及涉及的信号通路等进行分析;应用实时荧光定量PCR验证17个基因的差异表达。【结果】在感染24、48及72 h后,分别得到了9、51及258个差异表达基因。KEGG通路分析表明,在感染48及72 h后,大多数涉及到代谢通路的基因的表达水平下调,所有涉及到核糖体通路和蛋白酶体通路的基因的表达水平上调。一些免疫相关基因如丝氨酸蛋白酶抑制剂、脂酶、热激蛋白、细胞色素P450、核糖体P0蛋白等基因的表达水平上调。【结论】经荧光定量PCR验证的差异表达基因可能与家蚕感染质型多角体病毒的应答机制相关,研究结果为从分子水平上阐明家蚕感染质型多角体病毒的致病机理提供了新的研究方向。[ Objective ] The objective of this study is to screen the responsive gene of silkworm infected with Bombyx mori cytoplasmic polyhedrosis virus （BmCPV） and establish a foundation for elaborating the molecular mechanism of BmCPV infection. （Method ] A microarray system comprising about 23 000 oligonucluotide probes was employed to compare differentially expressed genes in the midguts of BmCPV-infected and normal silkworm larvae at 24, 48 and 72 h post-inoculation. Functions, classifications and pathways of these differentially expressed genes were analyzed by bioinformation tools. Seventeen differentially expressed genes were identified by quantitative real-time PCR. [Result] At 24, 48 and 72 h post-inoculation, 9, 51 and 258 differentially expressed genes were obtained, respectively. KEGG pathways analysis indicated that at 48 and 72 h post-inoculation, most genes related to metabolism pathways were down-regulated while expressions of genes involved in ribosome and proteasome pathway were all up-regulated. The expressions of several immune-related genes including serpin5, lipase, heat shock proteins, cytochrome P450s and ribosomal P0 protein were up-regulated. [Conclusion] Differentially expressed genes identified may be responsive to the BmCPV infection. The results provided new clues for investigating the molecular mechanism of BmCPV infection.

关 键 词：家蚕中肠 质型多角体病毒 基因芯片 应答基因 实时荧光定量PCR 

分 类 号：S884[农业科学—特种经济动物饲养]