补心方剂对小鼠外周血造血干细胞移植模型骨髓造血的影响  

Effects of Tonifying Heart Formulas on Medullary Hematopoiesis Function on Mouse Model of Peripheral Blood Stem Cell Transplantation

作　　者：刘渊[1] 谢晓红[2] 白蓝郦[1] 黄秀深[1] 

机构地区：[1]成都中医药大学基础医院,四川成都610075 [2]成都中医药大学附属医院,四川成都610072

出　　处：《成都中医药大学学报》2011年第2期52-53,74,共3页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(编号:81072728)

摘　　要：目的:观察补心方剂对外周血造血干细胞移植小鼠的影响。方法:将BALB/c小鼠随机分为正常对照组、模型组、单纯干细胞移植组、干细胞移植+炙甘草汤组、干细胞移植+天王补心丹组、干细胞移植+当归补血汤组、干细胞移植+参附汤组,每组25只。每组有10只用于观察死亡率,另15只用于检测血象及有核细胞数。结果:5天后观察小鼠死亡情况,以"干细胞移植+参附汤组"死亡率最低。给药各组对WBC、PLT、Hb、骨髓有核细胞数均有显著升高作用。结论:补心方剂对小鼠外周血造血干细胞移植模型早期骨髓重建及减少死亡有一定作用。Objective：To investigate the effects of tonifying heart formulas on the mouse model of peripheral blood stem cell transplantation.Methods：BALB/C mice were randomly divided into seven groups of 25 animals each ：normal control group,model group,stem cell transplantation group,the group of stem cell transplantation and Zhigancao soup,the group of stem cell transplantation and Tian wang bu xin Dan,the group of stem cell transplantation and Dang gui bu xue soup,the group of stem cell transplantation and Shenfu soup.In each group 10 rats were used for observing the mortality only,the rest of each group were examined on its hemogram and nucleated cells in bone marrow.Results：The group of stem cell transplantation and Shenfu soup had the lowest death rates within five day.The number of WBC、PLT、Hb and monocyte nucleated cells increased significantly in all the medicine groups.Conclusion： Tonifying heart formulas has certain effect on the earlier stage of marrow reconstruction on the mouse model of peripheral blood stem cell transplantation and mortality decreasing.

关 键 词：补心方剂 外周血造血干细胞移植 早期骨髓功能重建 

分 类 号：R285.5[医药卫生—中药学] R551[医药卫生—中医学]