Effects of nitrogen specification and culture method on growth of Enteromorpha prolifera  

作　　者：李俭平 赵卫红 

机构地区：[1]Key Laboratory of Marine Ecology & Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences [2]Graduate University of Chinese Academy of Sciences

出　　处：《Chinese Journal of Oceanology and Limnology》2011年第4期874-882,共9页中国海洋湖沼学报（英文版）

基　　金：Supported by the Major Projects of Knowledge Innovation Program of Chinese Academy of Sciences (No. KZCX2-SW-208-01);the National Natural Science Foundation of China (No. 40976047);National Basic Research Program of China (973 Program) (No. 2010CB428701)

摘　　要：Eutrophication, which is the enrichment of a water mass with inorganic and organic nutrients that support plant growth, is a key factor in stimulating phytoplankton growth. In this study, we determined the effects of various nitrogen sources, different nitrogen concentrations in the culture medium, and two culture methods on the growth of the green alga, Enteromorpha prolifera. The relationship between the specific growth rate of E. prolifera and NO3-N concentration was consistent with that estimated using the Monod equation （R2=0.9713, P〈0.01）. In the N O3-N medium, the maximum specific growth rate was calculated to be 0.1634/d and the semi-saturation constant was calculated to be 16.86 μmol/L. Our results show that E. prolijkra can effectively utilize NH4^＋-N, NO3^-N, and NO^2-N and urea-N in the range of 5 to 50 μmol/L. NH4^＋-N was preferentially assimilated by E. prolifera, and urea-N was favorable for long-term growth.Eutrophication, which is the enrichment of a water mass with inorganic and organic nutrients that support plant growth, is a key factor in stimulating phytoplankton growth. In this study, we determined the effects of various nitrogen sources, different nitrogen concentrations in the culture medium, and two culture methods on the growth of the green alga, Enteromorpha prolifera. The relationship between the specific growth rate of E. prolifera and NO3--N concentration was consistent with that estimated using the Monod equation (R2 = 0.9713, P < 0.01). In the NO3--N medium, the maximum specific growth rate was calculated to be 0.1634/d and the semi-saturation constant was calculated to be 16.86 μmol/L. Our results show that E. prolifera can effectively utilize NH4+-N, NO3--N, and NO2--N and urea-N in the range of 5 to 50 μmol/L. NH4+-N was preferentially assimilated by E. prolifera, and urea-N was favorable for long-term growth.

关 键 词：EUTROPHICATION N concentrations N sources culture methods E. prolifera 

分 类 号：X55[环境科学与工程—环境工程] X173